A direct comparison between extracted tooth and filter-membrane biofilm models of endodontic irrigation using Enterococcus faecalis

Hope, Christopher K ORCID: 0000-0003-2986-0824, Garton, Stuart G, Wang, Qian, Burnside, Girvan ORCID: 0000-0001-7398-1346 and Farrelly, Peter J (2010) A direct comparison between extracted tooth and filter-membrane biofilm models of endodontic irrigation using Enterococcus faecalis. ARCHIVES OF MICROBIOLOGY, 192 (9). pp. 775-781.

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Abstract

Endodontic restorations often fail due to inadequate disinfection of the root canal even though the antimicrobial irrigants used have been shown to be capable of killing the bacterium frequently implicated in this complication, Enterococcus faecalis (Ef). Extracted human teeth were root-prepared and filled with a liquid culture of Ef. Following incubation, the root canals were irrigated with 1% sodium hypochlorite (NaOCl), electrochemically activated water or saline control. Irrigation was modelled using an electronic pipette to deliver the solutions at a reproducible flow velocity. A series of parallel experiments employed a membrane biofilm model that was directly immersed into irrigant. Experimental conditions where contiguous between the extracted tooth model and biofilm model wherever possible. After 60 s of exposure, 1% NaOCl effectively sterilised the biofilm model, whereas log 3.36 viable Ef where recoverable from the analogous extracted tooth model, the other irrigants proved ineffective. Biofilms of Ef were susceptible to concentrations of irrigant that proved ineffective in the tooth model. NaOCl was the most effective biocide in either case. This suggests that the biofilm modality of bacterial growth may not be the most important factor for the recalcitrance of root canal infections during endodontic irrigation; it is more likely due to the inability of the irrigant to access the infection.

Item Type:	Article
Additional Information:	MATCHED FROM WOS ## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords:	Enterococcus faecalis, In vitro modelling, Hypochlorous acid, Sodium hypochlorite
Subjects:	?? RK ??
Divisions:	Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences > School of Dentistry
Depositing User:	Symplectic Admin
Date Deposited:	01 Sep 2010 09:39
Last Modified:	16 Dec 2022 04:34
DOI:	10.1007/s00203-010-0604-6
Publisher's Statement :	© Springer-Verlag 2010. This article was first published in Hope, Christopher K.; Garton, Stuart G.; Wang, Qian; Burnside, Girvan. and Farrelly, Peter J. (2010) A direct comparison between extracted tooth and filter-membrane biofilm models of endodontic irrigation using Enterococcus faecalis. Archives of Microbiology, 192 (9). pp. 775-781. http://dx.doi.org/10.1007/s00203-010-0604-6. The final publication is available at www.springerlink.com .
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/1386