Hybridization between Calopteryx splendens and C. haemorrhoidalis confirmed by morphological and genetic analyses


(2014) Hybridization between Calopteryx splendens and C. haemorrhoidalis confirmed by morphological and genetic analyses. International Journal of Odonatology, 17 (2-3). pp. 149-160. ISSN 2159-6719 (Online); 1388-7890 (Print)

WarningThere is a more recent version of this item available.
[img] Archive (Full-text PDF)
13887890.2014.951696 - Published Version
Access to this file is restricted: this item is under permanent embargo.
Download (55kB) | Request a copy

Abstract

Hybridization between Calopteryx haemorrhoidalis and any of its congeners has not been reported until now. We observed spontaneous matings between male C. splendens and female C. haemorrhoidalis at a locality in Central Italy, together with some putative hybrid individuals that had a mixed phenotype. Here, we report the morphological and molecular characterization of five suspected hybrids collected from this population during 2001 (n=1), 2012 (n=2) and 2013 (n=2). A discriminant analysis based on 13 morphological variables correctly separated both parental species (with 100% assignation success) and classified the hybrid from 2001 as splendens phenotype and those from 2012 and 2013 as haemorrhoidalis. Genotype data (microsatellite loci) was used to confirm the hybrid origin of these specimens, although there were differences between the individual from 2001 and those from 2012 and 2013; the 2001 individual had alleles that were present in both parent species, suggesting it is an F1 hybrid, but the individuals collected in 2012 and 2013 had private alleles at eight (out of 12) loci and only a small portion of the genome in common with C. splendens, which suggests that introgression is occurring in this population. Similarities in mitochondrial DNA sequences indicate that the 2001 hybrid and the 2012–2013 hybrids have splendens and haemorrhoidalis maternal origins respectively, which, in contrast with behavioural observations, indicates that interspecific matings in both directions are possible. This is the first demonstration that C. haemorrhoidalis can hybridize with other congeners to produce viable offspring.

Item Type: Article
Additional Information: Published online 23 Oct 2014.
Uncontrolled Keywords: Odonata, dragonfly; Calopterygidae; sympatric populations; discriminant analysis; microsatellites; hybrids; introgression
Subjects: Q Science > QH Natural history
Depositing User: Symplectic Admin
Date Deposited: 04 Nov 2014 12:23
Last Modified: 02 Nov 2018 10:19
DOI: 10.1080/13887890.2014.951696
URI: http://livrepository.liverpool.ac.uk/id/eprint/2000861

Available Versions of this Item

Repository Staff Access
Altmetric
CORE (COnnecting REpositories)