New EPIC nuclear DNA sequence markers to improve the resolution of phylogeographic studies of coenagrionids and other odonates

Ferreira, Sonia, Olalla Lorenzo-Carballa, M, Torres-Cambas, Yusdiel, Cordero-Rivera, Adolfo, Thompson, David J and Watts, Phillip C (2014) New EPIC nuclear DNA sequence markers to improve the resolution of phylogeographic studies of coenagrionids and other odonates. INTERNATIONAL JOURNAL OF ODONATOLOGY, 17 (2-3). pp. 135-147.

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Abstract

While phylogeographic data provide valuable information to inform conservation plans, there are comparatively few Odonata phylogeographic studies. This lack of research is partially due to a lack of independent DNA markers with appropriate levels of polymorphism that PCR-amplify in a range of species. We followed an exon-primed, intron-crossing (EPIC) PCR strategy to develop five new, polymorphic nuclear DNA sequence loci (six distinct DNA fragments) for the southern damselfly Coenagrion mercuriale. These markers were: cell division cycle 5 protein (CDC5), arginine methyltransferase (PRMT), acetylglucosaminyl-transferase (AgT), myosin light chain (MLC) and phosphoglucose isomerase (PGI). Between three and five of these new markers could be PCR-amplified in five other species from the genus Coenagrion; one locus (PRMT) can be used in 26 other species of odonates that we examined, including three species of Anisoptera belonging to the genus Onychogomphus. These new nuclear genetic markers will be useful for phylogeographic studies in a range of odonate species, but also for phylogenetic studies, providing a particularly useful complement to the existing mitochondrial and nuclear loci.

Item Type:	Article
Additional Information:	## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords:	phylogeny, multilocus approach, Odonata, nuclear DNA loci, introns, Coenagrion mercuriale, dragonfly, Coenagrionidae, genetic diversity, non-model organism
Subjects:	?? QL ??
Depositing User:	Symplectic Admin
Date Deposited:	04 Nov 2014 16:45
Last Modified:	16 Mar 2024 06:41
DOI:	10.1080/13887890.2014.950698
Related URLs:	Author
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2000864