Inhibition of tumourigenicity of small cell lung cancer cells by suppressing Id3 expression.

Kamalian, Laleh, Forootan, Shiva S, Bao, Zheng Z, Zhang, Yu, Gosney, John R, Foster, Christopher S and Ke, Youqiang (2010) Inhibition of tumourigenicity of small cell lung cancer cells by suppressing Id3 expression. PhD thesis, University of Liverpool.

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Abstract

Id3 is over-expressed in small cell lung cancer (SCLC). To test whether the tumourigenicity of SCLC cells can be inhibited by suppressing Id3 expression, we transfected siRNA into SCLC cell line GLC-19 and established two sublines (G-Id3-1 and G-Id3-7) which expressed only 30% of the level of Id3 measured in control transfectants. Suppression of Id3 expression in both G-Id3-1 and G-Id3-7 cells produced significant reductions in proliferation rates and in numbers of colonies formed in soft agar assay. When G-Id3-1, G-Id3-7 and the control transfectants were inoculated subcutaneously into 3 groups (8 each) of nude mice, respectively, all (100%) inoculated animals produced tumours. Although there was no difference in tumour incidents amongst the 3 groups, significant reductions were observed in both size and weight of tumours produced by either G-Id3-1 or G-Id3-7 cells. While the final average volume of tumours produced in control group was 1012.1+/-394 mm(3), it was significantly reduced (p<0.001, p<0.01) by 2.1- and 2.9-fold to 475.7+/-167 mm(3) and 354.3+/-218 mm(3) in groups inoculated with G-Id3-1 and G-Id3-7 cells, respectively. Similar differences were also observed in average weight of tumours. Upon induction of apoptosis by cytotoxin camptothecin, the percentages of apoptotic cells in G-Id3-1 and G-Id3-7 were, respectively >2.4-fold higher than that in control. The results in this study suggest that highly expressed Id3 in SCLC cells may be an important therapeutic target for tumour suppression.

Item Type:	Thesis (PhD)
Additional Information:	Date: 2014-06 (completed)
Uncontrolled Keywords:	Cell Line, Tumor, Animals, Humans, Mice, Mice, Nude, Lung Neoplasms, Camptothecin, Neoplasm Proteins, RNA, Small Interfering, Transfection, Cell Growth Processes, Gene Expression Regulation, Neoplastic, Base Sequence, Molecular Sequence Data, Inhibitor of Differentiation Proteins, Small Cell Lung Carcinoma
Depositing User:	Symplectic Admin
Date Deposited:	22 Jul 2015 15:21
Last Modified:	17 Dec 2022 01:38
DOI:	10.17638/02003479
Related URLs:	Author
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2003479