Palaeosymbiosis Revealed by Genomic Fossils of Wolbachia in a Strongyloidean Nematode

Koutsovoulos, Georgios, Makepeace, Ben ORCID: 0000-0002-6100-6727, Ngwang, Tanya and Blaxter, Mark (2014) Palaeosymbiosis Revealed by Genomic Fossils of Wolbachia in a Strongyloidean Nematode. PLoS Genetics, 10 (6). e1004397-.

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Abstract

Wolbachia are common endosymbionts of terrestrial arthropods, and are also found in nematodes: the animal-parasitic filaria, and the plant-parasite Radopholus similis. Lateral transfer of Wolbachia DNA to the host genome is common. We generated a draft genome sequence for the strongyloidean nematode parasite Dictyocaulus viviparus, the cattle lungworm. In the assembly, we identified nearly 1 Mb of sequence with similarity to Wolbachia. The fragments were unlikely to derive from a live Wolbachia infection: most were short, and the genes were disabled through inactivating mutations. Many fragments were co-assembled with definitively nematode-derived sequence. We found limited evidence of expression of the Wolbachia-derived genes. The D. viviparus Wolbachia genes were most similar to filarial strains and strains from the host promiscuous clade F. We conclude that D. viviparus was infected by Wolbachia in the past, and that clade F-like symbionts may have been the source of filarial Wolbachia infections.

Item Type:	Article
Uncontrolled Keywords:	Animals, Cattle, Dictyocaulus, Wolbachia, Cattle Diseases, DNA, Bacterial, DNA, Protozoan, Sequence Analysis, DNA, Symbiosis, Base Sequence
Depositing User:	Symplectic Admin
Date Deposited:	16 Mar 2015 16:59
Last Modified:	15 Dec 2022 10:11
DOI:	10.1371/journal.pgen.1004397
Publisher's Statement :	© 2014 Koutsovoulos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2008197