Divergent, CoexistingPseudomonas aeruginosaLineages in Chronic Cystic Fibrosis Lung Infections

Williams, David, Evans, Benjamin, Haldenby, Sam, Walshaw, Martin J, Brockhurst, Michael A, Winstanley, Craig ORCID: 0000-0002-2662-8053 and Paterson, Steve ORCID: 0000-0002-1307-2981 (2015) Divergent, CoexistingPseudomonas aeruginosaLineages in Chronic Cystic Fibrosis Lung Infections. American Journal of Respiratory and Critical Care Medicine, 191 (7). pp. 775-785.

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Abstract

Rationale Pseudomonas aeruginosa, the predominant cause of chronic airway infections of cystic fibrosis patients, exhibits extensive phenotypic diversity among isolates within and between sputum samples, but little is known about the underlying genetic diversity. Objectives Our aim was to characterise the population genetic structure of transmissible P. aeruginosa Liverpool Epidemic Strain in chronic infections of nine cystic fibrosis patients, and to infer the evolutionary processes by which P. aeruginosa populations adapt to the cystic fibrosis lung. Methods We performed whole genome sequencing of Pseudomonas aeruginosa isolates and pooled populations and used comparative analyses of genome sequences, including population genomics and phylogenetic reconstructions, and resolution of population structure from genome-wide allele frequencies. Measurements and Main Results Genome sequences were obtained for 360 isolates from nine patients. Phylogenetic reconstruction of 40 individually sequenced isolates from one patient sputum sample revealed the co-existence of two genetically diverged, recombining lineages exchanging potentially adaptive mutations. Analysis of population samples for eight additional patients indicated co-existing lineages in six cases. Phylogenetic reconstruction of individually sequenced isolates from all nine patients indicated greater evolutionary distances within than between patients in most cases.

Item Type:	Article
Subjects:	?? QR ??
Depositing User:	Symplectic Admin
Date Deposited:	23 Jun 2015 07:58
Last Modified:	17 Dec 2022 01:40
DOI:	10.1164/rccm.201409-1646OC
Related URLs:	Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2014379