Serum Mannose-Binding Lectin Concentration, but Not Genotype, Is Associated With Clostridium difficile Infection Recurrence: A Prospective Cohort Study

Swale, Andrew, Miyajima, Fabio, Kolamunnage-Dona, Ruwanthi ORCID: 0000-0003-3886-6208, Roberts, Paul, Little, Margaret, Beeching, Nick ORCID: 0000-0002-7019-8791, Beadsworth, Michael, Liloglou, Lakis ORCID: 0000-0003-0460-1404 and Pirmohamed, Munir ORCID: 0000-0002-7534-7266 (2014) Serum Mannose-Binding Lectin Concentration, but Not Genotype, Is Associated With Clostridium difficile Infection Recurrence: A Prospective Cohort Study. Clinical Infectious Diseases, 59 (10). pp. 1429-1436.

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Abstract

Background. Mannose-binding lectin (MBL) plays a key role in the activation of the lectin-complement pathway of innate immunity, and its deficiency has been linked with several acute infections. However, its role in predisposing to, or modulating disease severity in, Clostridium difficile infection (CDI) has not been investigated. Methods. We prospectively recruited 308 CDI case patients and 145 control patients with antibiotic-associated diarrhea (AAD). CDI outcome measures were disease severity, duration of symptoms, 30-day mortality, and 90-day recurrence. Serum concentrations of MBL were determined using a commercial enzyme-linked immunosorbent assay transferred to an electrochemiluminescence–based platform. MBL2 polymorphisms were typed using a combination of pyrosequencing and TaqMan genotyping assays. Results. The frequency of the MBL2 genetic variants was similar to that reported in other white populations. MBL serum concentrations in CDI and AAD subjects were determined by MBL2 exonic variants B, C, and D and the haplotypes (LYPB, LYQC, and HYPD). There was no difference in either MBL concentrations or genotypes between cases and controls. MBL concentration, but not genotype, was a determinant of CDI recurrence (odds ratios, 3.18 [95% confidence interval {CI}, 1.40–7.24] and 2.61 [95% CI, 1.35–5.04] at the

Item Type:	Article
Uncontrolled Keywords:	Clostridium difficile, CDI, MBL, Disease recurrance
Depositing User:	Symplectic Admin
Date Deposited:	22 Jul 2015 10:52
Last Modified:	15 Dec 2022 11:41
DOI:	10.1093/cid/ciu666
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2016882