Nyirenda, Tonney S, Gilchrist, James J, Feasey, Nicholas A, Glennie, Sarah J, Bar-Zeev, Naor ORCID: 0000-0003-0570-4624, Gordon, Melita ORCID: 0000-0002-0629-0884, MacLennan, Calman A, Mandala, Wilson L and Heyderman, Robert S
(2014)
Sequential Acquisition of T Cells and Antibodies to Nontyphoidal Salmonella in Malawian Children.
The Journal of Infectious Diseases, 210 (1).
pp. 56-64.
Text
J Infect Dis.-2014-Nyirenda-56-64 published.pdf - Unspecified Download (648kB) |
Abstract
Background. Salmonella Typhimurium (STm) remain a prominent cause of bacteremia in sub-Saharan Africa. Complement-fixing antibodies to STm develop by 2 years of age. We hypothesized that STm-specific CD4+ T cells develop alongside this process. Methods. Eighty healthy Malawian children aged 0–60 months were recruited. STm-specific CD4+ T cells producing interferon γ, tumor necrosis factor α, and interleukin 2 were quantified using intracellular cytokine staining. Antibodies to STm were measured by serum bactericidal activity (SBA) assay, and anti-STm immunoglobulin G antibodies by enzyme-linked immunosorbent assay. Results. Between 2006 and 2011, STm bacteremias were detected in 449 children <5 years old. STm-specific CD4+ T cells were acquired in infancy, peaked at 14 months, and then declined. STm-specific SBA was detectable in newborns, declined in the first 8 months, and then increased to a peak at age 35 months. Acquisition of SBA correlated with acquisition of anti–STm–lipopolysaccharide (LPS) immunoglobulin G (r = 0.329 [95% confidence interval, .552–.062]; P = .01) but not anti–STm–outer membrane protein or anti–STm-flagellar protein (FliC). Conclusions. Acquisition of STm-specific CD4+ T cells in early childhood is consistent with early exposure to STm or cross-reactive protein antigens priming this T-cell development. STm-specific CD4+ T cells seem insufficient to protect against invasive nontyphoidal Salmonella disease, but sequential acquisition of SBA to STm LPS is associated with a decline in its incidence.
Item Type: | Article |
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Uncontrolled Keywords: | Salmonella, T cell, antibody, cytokine, children |
Subjects: | ?? R1 ?? |
Depositing User: | Symplectic Admin |
Date Deposited: | 15 Sep 2015 08:12 |
Last Modified: | 16 Dec 2022 16:50 |
DOI: | 10.1093/infdis/jiu045 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/2025667 |