Preventing Plasmon Coupling between Gold Nanorods Improves the Sensitivity of Photoacoustic Detection of Labeled Stem Cells in Vivo



Comenge, J, Fragueiro, O ORCID: 0000-0002-9818-7453, Sharkey, J, Taylor, A ORCID: 0000-0003-2028-6694, Held, M ORCID: 0000-0003-0118-5898, Burton, NC, Park, BK ORCID: 0000-0001-8384-824X, Wilm, B ORCID: 0000-0002-9245-993X, Murray, P, Brust, M ORCID: 0000-0001-6301-7123
et al (show 1 more authors) (2016) Preventing Plasmon Coupling between Gold Nanorods Improves the Sensitivity of Photoacoustic Detection of Labeled Stem Cells in Vivo. ACS Nano, 10 (7). pp. 7106-7116.

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Abstract

© 2016 American Chemical Society.Gold nanorods are excellent contrast agents for imaging technologies which rely on near-infrared absorption such as photoacoustic imaging. For cell tracking applications, the cells of interest are labeled with the contrast agent prior to injection. However, after uptake into cells by endocytosis, the confinement and high concentration in endosomes leads to plasmon band broadening and reduced absorbance. This would limit the potential of multispectral optoacoustic tomography in terms of spectral processing and, consequently, sensitivity. Here, we show that steric hindrance provided by silica coating of the nanorods leads to the preservation of their spectral properties and improved photoacoustic sensitivity. This strategy allowed the detection and monitoring of as few as 2 × 104 mesenchymal stem cells in mice over a period of 15 days with a high spatial resolution. Importantly, the silica-coated nanorods did not affect the viability or differentiation potential of the transplanted mesenchymal stem cells.

Item Type: Article
Uncontrolled Keywords: photophysics, absorption, silica, cells, plasmons
Depositing User: Symplectic Admin
Date Deposited: 24 Jun 2016 08:02
Last Modified: 19 Jan 2023 07:35
DOI: 10.1021/acsnano.6b03246
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3001829