Characterisation of cultured mesothelial cells derived from the murine adult omentum

Dauleh, S, Santeramo, I, Fielding, C, Ward, K, Herrmann, A ORCID: 0000-0002-0858-419X, Murray, PM and Wilm, B ORCID: 0000-0002-9245-993X (2016) Characterisation of cultured mesothelial cells derived from the murine adult omentum. PLoS ONE, 11 (7). e0158997-.

Text PONE-D-15-56276R2_FTC.pdf - Author Accepted Manuscript Download (286kB)

Abstract

The human omentum has been long regarded as a healing patch, used by surgeons for its ability to immunomodulate, repair and vascularise injured tissues. A major component of the omentum are mesothelial cells, which display some of the characteristics of mesenchymal stem/stromal cells. For instance, lineage tracing studies have shown that mesothelial cells give rise to adipocytes and vascular smooth muscle cells, and human and rat mesothelial cells have been shown to differentiate into osteoblast- and adipocyte-like cells in vitro, indicating that they have considerable plasticity. However, so far, long-term cultures of mesothelial cells have not been successfully established due to early senescence. Here, we demonstrate that mesothelial cells isolated from the mouse omentum could be cultured for more than 30 passages. While epithelial markers were downregulated over passages in the mesothelial cells, their mesenchymal profile remained unchanged. Early passage mesothelial cells displayed clonogenicitiy, expressed several stem cell markers, and up to passage 5 and 13, respectively, could differentiate along the adipogenic and osteogenic lineages, demonstrating stem/progenitor characteristics and differentiation potential.

Item Type:	Article
Uncontrolled Keywords:	Omentum, Cells, Cultured, Adipocytes, Osteoblasts, Epithelial Cells, Mesenchymal Stem Cells, Animals, Mice, Cell Differentiation, Gene Expression Regulation, Cell Lineage, Osteogenesis, Pregnancy, Female, Adipogenesis, Biomarkers
Depositing User:	Symplectic Admin
Date Deposited:	04 Jul 2016 07:36
Last Modified:	19 Jan 2023 07:34
DOI:	10.1371/journal.pone.0158997
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3002054