Human limbal mesenchymal stem cells express ABCB5 and can grow on amniotic membrane

Shaharuddin, B, Osei-Bempong, C, Ahmad, S, Rooney, P, Ali, S, Oldershaw, R ORCID: 0000-0001-8478-599X and Meeson, A (2016) Human limbal mesenchymal stem cells express ABCB5 and can grow on amniotic membrane. REGENERATIVE MEDICINE, 11 (03). pp. 273-286.

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Abstract

Aim: To isolate and characterize limbal mesenchymal stem cells (LMSCs) from human corneoscleral rings. Materials & methods: Cells were isolated from corneoscleral rings and cultured in a mesenchymal stem cell (MSC)-selective media and examined for differentiation, phenotyping and characterization. Results: LMSCs were capable of trilineage differentiation, adhered to tissue culture plastic, expressed HLA class I and cell surface antigens associated with human MSC while having no/low expression of HLA class II and negative hematopoietic lineage markers. They were capable for CXCL12-mediated cellular migration. LMSCs adhered, proliferated on amniotic membrane and expressed the common putative limbal stem cell markers. Conclusion: Limbal-derived MSC exhibited plasticity, could maintain limbal markers expression and demonstrated viable growth on amniotic membrane.

Item Type:	Article
Uncontrolled Keywords:	ABCB5, amniotic membrane, limbal mesenchymal stem cells, limbal stem cells, limbal stem cell deficiency
Depositing User:	Symplectic Admin
Date Deposited:	27 Jul 2016 14:54
Last Modified:	19 Jan 2023 07:32
DOI:	10.2217/rme-2016-0009
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3002575