A preliminary study of the effects of pH upon fluorescence in suspensions of prevotella intermedia

Hope, CK ORCID: 0000-0003-2986-0824, Billingsley, K, De Jong, EJD and Higham, SM ORCID: 0000-0002-4097-4702 (2016) A preliminary study of the effects of pH upon fluorescence in suspensions of prevotella intermedia. PLoS ONE, 11 (07).

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Abstract

© 2016 Hope et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.The quantification of fluorescence in dental plaque is currently being developed as a diagnostic tool to help inform and improve oral health. The oral anaerobe Prevotella intermedia exhibits red fluorescence due to the accumulation of porphyrins. pH affects the fluorescence of abiotic preparations of porphyrins caused by changes in speciation between monomers, higher aggregates and dimers, but this phenomenon has not been demonstrated in bacteria. Fluorescence spectra were obtained from suspensions of P. intermedia that were adjusted to pHs commensurate with the range found within dental plaque. Two fluorescent motifs were identified; 410 nm excitation / 634 nm emission (peak A) and 398 nm excitation / 622 nm emission (peak B). A transition in the fluorescence spectra was observed from peak A to peak B with increasing pH which was also evident as culture age increased from 24 hours to 96 hours. In addition to these 'blue-shifts', the intensity of peak A increased with pH whilst decreasing with culture age from 24 to 96 hours. A bacterium's relationship with the local physiochemical environment at the time of image capture may therefore affect the quantification of dental plaque fluorescence.

Item Type:	Article
Uncontrolled Keywords:	Prevotella intermedia, Spectrometry, Fluorescence, Hydrogen-Ion Concentration, Fluorescence, Time Factors
Depositing User:	Symplectic Admin
Date Deposited:	08 Aug 2016 07:25
Last Modified:	19 Jan 2023 07:32
DOI:	10.1371/journal.pone.0158835
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3002800