MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection



Gao, Jiming, Xiao, Shuqi, Xiao, Yihong, Wang, Xiangpeng, Zhang, Chong, Zhao, Qin, Nan, Yuchen, Huang, Baicheng, Liu, Hongliang, Liu, Ningning
et al (show 10 more authors) (2016) MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection. SCIENTIFIC REPORTS, 6 (1). 25120-.

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Abstract

Porcine reproductive and respiratory syndrome (PRRS) caused by the PRRS virus (PRRSV) is an important swine disease worldwide. PRRSV has a limited tropism for certain cells, which may at least in part be attributed to the expression of the necessary cellular molecules serving as the virus receptors or factors on host cells for virus binding or entry. However, these molecules conferring PRRSV infection have not been fully characterized. Here we show the identification of non-muscle myosin heavy chain 9 (MYH9) as an essential factor for PRRSV infection using the anti-idiotypic antibody specific to the PRRSV glycoprotein GP5. MYH9 physically interacts with the PRRSV GP5 protein via its C-terminal domain and confers susceptibility of cells to PRRSV infection. These findings indicate that MYH9 is an essential factor for PRRSV infection and provide new insights into PRRSV-host interactions and viral entry, potentially facilitating development of control strategies for this important swine disease.

Item Type: Article
Uncontrolled Keywords: Cell Line, Animals, Swine, Porcine respiratory and reproductive syndrome virus, Nonmuscle Myosin Type IIA, Viral Envelope Proteins, Protein Interaction Mapping, Protein Binding, Host-Pathogen Interactions
Depositing User: Symplectic Admin
Date Deposited: 28 Oct 2016 08:55
Last Modified: 19 Jan 2023 07:27
DOI: 10.1038/srep25120
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3004174