A new broccoli x broccoli immortal mapping population and framework genetic map: tools for breeders and complex trait analysis

Walley, Peter Glen ORCID: 0000-0001-9166-6294, Carder, John, Skipper, Emma, Mathas, Evy, Lynn, James, Pink, David and Buchanan-Wollaston, Vicky (2012) A new broccoli x broccoli immortal mapping population and framework genetic map: tools for breeders and complex trait analysis. THEORETICAL AND APPLIED GENETICS, 124 (3). pp. 467-484.

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Official URL: http://dx.doi.org/10.1007/s00122-011-1721-6

Abstract

A unique broccoli × broccoli doubled haploid (DH) population has been created from the F(1) of a cross between two DH broccoli lines derived from cultivars Green Duke and Marathon. We genotyped 154 individuals from this population with simple sequence repeat and amplified fragment length polymorphism markers to create a B. oleracea L. var. italica 'intra-crop' specific framework linkage map. The map is composed of nine linkage groups with a total length of 946.7 cM. Previous published B. oleracea maps have been constructed using diverse crosses between morphotypes of B. oleracea; this map therefore represents a useful breeding resource for the dissection of broccoli specific traits. Phenotype data have been collected from the population over five growing seasons; the framework linkage map has been used to locate quantitative trait loci for agronomically important broccoli traits including head weight (saleable yield), head diameter, stalk diameter, weight loss and relative weight loss during storage, as well as traits for broccoli leaf architecture. This population and associated linkage map will aid breeders to directly map agronomically important traits for the improvement of elite broccoli cultivars.

Item Type:	Article
Uncontrolled Keywords:	Brassica, Plant Leaves, DNA Primers, Linear Models, Chromosome Mapping, Crosses, Genetic, Breeding, Species Specificity, Microsatellite Repeats, Genotype, Phenotype, Quantitative Trait Loci, Amplified Fragment Length Polymorphism Analysis
Depositing User:	Symplectic Admin
Date Deposited:	01 Nov 2016 16:38
Last Modified:	19 Jan 2023 07:26
DOI:	10.1007/s00122-011-1721-6
Open Access URL:	http://link.springer.com/article/10.1007/s00122-01...
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3004251