Defective Mitochondrial Function <i>In Vivo</i> in Skeletal Muscle in Adults with Down's Syndrome: A <SUP>31</SUP>P-MRS Study

Phillips, Alexander C, Sleigh, Alison, McAllister, Catherine J, Brage, Soren, Carpenter, T Adrian, Kemp, Graham J ORCID: 0000-0002-8324-9666 and Holland, Anthony J (2013) Defective Mitochondrial Function <i>In Vivo</i> in Skeletal Muscle in Adults with Down's Syndrome: A <SUP>31</SUP>P-MRS Study. PLOS ONE, 8 (12). e84031-.

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Abstract

Down's syndrome (DS) is a developmental disorder associated with intellectual disability (ID). We have previously shown that people with DS engage in very low levels of exercise compared to people with ID not due to DS. Many aspects of the DS phenotype, such as dementia, low activity levels and poor muscle tone, are shared with disorders of mitochondrial origin, and mitochondrial dysfunction has been demonstrated in cultured DS tissue. We undertook a phosphorus magnetic resonance spectroscopy ((31)P-MRS) study in the quadriceps muscle of 14 people with DS and 11 non-DS ID controls to investigate the post-exercise resynthesis kinetics of phosphocreatine (PCr), which relies on mitochondrial respiratory function and yields a measure of muscle mitochondrial function in vivo. We found that the PCr recovery rate constant was significantly decreased in adults with DS compared to non-DS ID controls (1.7 ± 0.1 min(-1) vs 2.1 ± 0.1 min(-1) respectively) who were matched for physical activity levels, indicating that muscle mitochondrial function in vivo is impaired in DS. This is the first study to investigate mitochondrial function in vivo in DS using (31)P-MRS. Our study is consistent with previous in vitro studies, supporting a theory of a global mitochondrial defect in DS.

Item Type:	Article
Additional Information:	## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords:	Muscle, Skeletal, Mitochondria, Muscle, Humans, Down Syndrome, Phosphorus Radioisotopes, Phosphocreatine, Exercise, Magnetic Resonance Spectroscopy, Case-Control Studies, Energy Metabolism, Kinetics, Adult, Female, Male, Intellectual Disability
Depositing User:	Symplectic Admin
Date Deposited:	13 Dec 2016 10:22
Last Modified:	10 Oct 2023 21:23
DOI:	10.1371/journal.pone.0084031
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3004840