A dielectrophoresis-impedance method for protein detection and analysis

Mohamad, Ahmad Sabry, Hamzah, Roszymah, Hoettges, Kai F ORCID: 0000-0002-0415-1688 and Hughes, Michael Pycraft (2017) A dielectrophoresis-impedance method for protein detection and analysis. AIP ADVANCES, 7 (1). 015202-015202.

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Abstract

<jats:p>Dielectrophoresis (DEP) has increasingly been used for the assessment of the electrical properties of molecular scale objects including proteins, DNA, nanotubes and nanowires. However, whilst techniques have been developed for the electrical characterisation of frequency-dependent DEP response, biomolecular study is usually limited to observation using fluorescent markers, limiting its applicability as a characterisation tool. In this paper we present a label-free, impedance-based method of characterisation applied to the determination of the electrical properties of colloidal protein molecules, specifically Bovine Serum Albumin (BSA). By monitoring the impedance between electrodes as proteins collect, it is shown to be possible to observe multi-dispersion behaviour. A DEP dispersion exhibited at 400 kHz is attributable to the orientational dispersion of the molecule, whilst a second, higher-frequency dispersion is attributed to a Maxwell-Wagner type dispersion; changes in behaviour with medium conductivity suggest that this is strongly influenced by the electrical double layer surrounding the molecule.</jats:p>

Item Type:	Article
Uncontrolled Keywords:	Generic health relevance
Depositing User:	Symplectic Admin
Date Deposited:	09 Feb 2017 11:42
Last Modified:	15 Mar 2024 02:54
DOI:	10.1063/1.4974290
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3005662