del Carmen Munoz-Marin, Maria, Gomez-Baena, Guadalupe 
ORCID: 0000-0003-3796-3874, Diez, Jesus, Beynon, Robert J ORCID: 0000-0003-0857-495X, Gonzalez-Ballester, David, Zubkov, Mikhail V and Garcia-Fernandez, Jose M
(2017)
Glucose Uptake in Prochlorococcus: Diversity of Kinetics and Effects on the Metabolism.
FRONTIERS IN MICROBIOLOGY, 8 (MAR).
327-.
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Glucose Uptake in Prochlorococcus: Diversity of Kinetics and Effects on the Metabolism.pdf - Published version Download (5MB) |
Abstract
We have previously shown that <i>Prochlorococcus</i> sp. SS120 strain takes up glucose by using a multiphasic transporter encoded by the Pro1404 gene. Here, we studied the glucose uptake kinetics in multiple <i>Prochlorococcus</i> strains from different ecotypes, observing diverse values for the K<sub>s</sub> constants (15-126.60 nM) and the uptake rates (0.48-6.36 pmol min<sup>-1</sup> mg prot<sup>-1</sup>). Multiphasic kinetics was observed in all studied strains, except for TAK9803-2. Pro1404 gene expression studies during the 21st Atlantic Meridional Transect cruise showed positive correlation with glucose concentrations in the ocean. This suggests that the Pro1404 transporter has been subjected to diversification along the <i>Prochlorococcus</i> evolution, in a process probably driven by the glucose availabilities at the different niches it inhabits. The glucose uptake mechanism seems to be a primary transporter. Glucose addition induced detectable transcriptomic and proteomic changes in <i>Prochlorococcus</i> SS120, but photosynthetic efficiency was unaffected. Our studies indicate that glucose is actively taken up by <i>Prochlorococcus</i>, but its uptake does not significantly alter the trophic ways of this cyanobacterium, which continues performing photosynthesis. Therefore <i>Prochlorococcus</i> seems to remain acting as a fundamentally phototrophic organism, capable of using glucose as an extra resource of carbon and energy when available in the environment.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | marine cyanobacteria, mixotrophy, transporter kinetics, proteomics, gene expression |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 07 Jun 2017 09:18 |
| Last Modified: | 19 Jan 2023 07:03 |
| DOI: | 10.3389/fmicb.2017.00327 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3007844 |
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