The Wnt5a receptor ROR2 is a predictive cell surface marker of human mesenchymal stem cells with an enhanced capacity for chondrogenic differentiation.

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Dickinson, SC, Sutton, CA, Brady, K, Salerno, A, Katopodi, T, Williams, RL, West, CC, Evseenko, D, Wu, L, Pang, S et al (show 6 more authors) , Ferro de Godoy, R, Goodship, AE, Péault, B, Blom, AW, Kafienah, W and Hollander, AP (2017) The Wnt5a receptor ROR2 is a predictive cell surface marker of human mesenchymal stem cells with an enhanced capacity for chondrogenic differentiation. Stem Cells. ISSN 1549-4918

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Abstract

Multipotent mesenchymal stem cells (MSCs) have enormous potential in tissue engineering and regenerative medicine. However until now their development for clinical use has been severely limited as they are a mixed population of cells with varying capacities for lineage differentiation and tissue formation. Here we identify ROR2 as a cell surface marker expressed by those MSCs with an enhanced capacity for cartilage formation. We generated clonal human MSC populations with varying capacities for chondrogenesis. ROR2 was identified through screening for upregulated genes in the most chondrogenic clones. When isolated from un-cloned populations, ROR2+ve MSCs were significantly more chondrogenic than either ROR2-ve or unfractionated MSCs. In a sheep cartilage-repair model they produced significantly more defect filling with no loss of cartilage quality compared with controls. ROR2+ve MSCs/perivascular cells were present in developing human cartilage, adult bone marrow and adipose tissue. Their frequency in bone marrow was significantly lower in patients with osteoarthritis than in controls. However after isolation of these cells and their initial expansion in vitro, there was greater ROR2 expression in the population derived from osteoarthritis patients compared with controls. Furthermore, osteoarthritis-derived MSCs were better able to form cartilage than MSCs from control patients in a tissue engineering assay. We conclude that MSCs expressing high levels of ROR2 provide a defined population capable of predictably enhanced cartilage production. This article is protected by copyright. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	Arthritis, Cell Signaling, Cellular therapy, Mesenchymal stem cells, Tissue engineering
Depositing User:	Symplectic Admin
Date Deposited:	23 Aug 2017 06:13
Last Modified:	19 Jan 2023 06:57
DOI:	10.1002/stem.2691
Related URLs:	https://www.ncbi.nlm.nih.gov/pubmed/2883...
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3009096

Available Versions of this Item

• The Wnt5a receptor ROR2 is a predictive cell surface marker of human mesenchymal stem cells with an enhanced capacity for chondrogenic differentiation. (deposited 16 Aug 2017 07:24)
  • The Wnt5a receptor ROR2 is a predictive cell surface marker of human mesenchymal stem cells with an enhanced capacity for chondrogenic differentiation. (deposited 23 Aug 2017 06:13) [Currently Displayed]
    • The Wnt5a Receptor, Receptor Tyrosine Kinase-Like Orphan Receptor 2, Is a Predictive Cell Surface Marker of Human Mesenchymal Stem Cells with an Enhanced Capacity for Chondrogenic Differentiation. (deposited 01 Sep 2017 10:04)