The Role of Controlled Surface Topography and Chemistry on Mouse Embryonic Stem Cell Attachment, Growth and Self-Renewal

Macgregor, Melanie, Williams, Rachel ORCID: 0000-0002-1954-0256, Downes, Joni, Bachhuka, Akash and Vasilev, Krasimir (2017) The Role of Controlled Surface Topography and Chemistry on Mouse Embryonic Stem Cell Attachment, Growth and Self-Renewal. MATERIALS, 10 (9). E1081-.

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Official URL: http://dx.doi.org/10.3390/ma10091081

Abstract

The success of stem cell therapies relies heavily on our ability to control their fate in vitro during expansion to ensure an appropriate supply. The biophysical properties of the cell culture environment have been recognised as a potent stimuli influencing cellular behaviour. In this work we used advanced plasma-based techniques to generate model culture substrates with controlled nanotopographical features of 16 nm, 38 nm and 68 nm in magnitude, and three differently tailored surface chemical functionalities. The effect of these two surface properties on the adhesion, spreading, and self-renewal of mouse embryonic stem cells (mESCs) were assessed. The results demonstrated that physical and chemical cues influenced the behaviour of these stem cells in in vitro culture in different ways. The size of the nanotopographical features impacted on the cell adhesion, spreading and proliferation, while the chemistry influenced the cell self-renewal and differentiation.

Item Type:	Article
Uncontrolled Keywords:	plasma polymer, Polyallylamine, polyoctadiene, polyacrylic acid, nanotopography, Mouse embryonic stem cells, fibronectin, Extra Cellular Matrix physical cues, Extra Cellular Matrix chemical cues
Depositing User:	Symplectic Admin
Date Deposited:	14 Sep 2017 12:38
Last Modified:	19 Jan 2023 06:54
DOI:	10.3390/ma10091081
Open Access URL:	http://www.mdpi.com/1996-1944/10/9/1081
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3009469