Cytotoxic and Antimicrobial Constituents from the Essential Oil of Lippia alba (Verbenaceae).

Santos, Nara O Dos, Pascon, Renata C, Vallim, Marcelo A ORCID: 0000-0002-6929-0566, Figueiredo, Carlos R ORCID: 0000-0003-3680-4070, Soares, Marisi G, Lago, João Henrique G ORCID: 0000-0002-1193-8374 and Sartorelli, Patricia (2016) Cytotoxic and Antimicrobial Constituents from the Essential Oil of Lippia alba (Verbenaceae). Medicines (Basel, Switzerland), 3 (3). E22-.

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Abstract

<b>Backgroud:</b><i>Lippia alba</i> (Verbenaceae) is a plant widely used in folk medicine to treat various diseases. The present work deals with the chemical composition of the crude essential oil extracted from leaves of <i>L. alba</i> and evaluation of its antimicrobial and cytotoxic activities. <b>Methods:</b> Leaves of <i>L. alba</i> were extracted by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC/MS) as well as by nuclear magnetic resonance (NMR) spectroscopy. Cytotoxic and antimicrobial activities of crude essential oil were evaluated in vitro using MTT and broth microdilution assays, respectively. <b>Results:</b> Chemical analysis afforded the identification of 39 substances corresponding to 99.45% of the total oil composition. Concerning the main compounds, monoterpenes nerol/geraniol and citral correspond to approximately 50% of crude oil. The cytotoxic activity of obtained essential oil against several tumor cell lines showed IC₅₀ values ranging from 45 to 64 µg/mL for B16F10Nex2 (murine melanoma) and A549 (human lung adenocarcinoma). In the antimicrobial assay, was observed that all tested yeast strains, except <i>C. albicans</i>, were sensitive to crude essential oil. MIC values were two to four-folds lower than those determined to bacterial strains. <b>Conclusion:</b> Analysis of chemical composition of essential oils from leaves of <i>L. alba</i> suggested a new chemotype nerol/geraniol and citral. Based in biological evidences, a possible application for studied oil as an antifungal in medicine, as well as in agriculture, is described.

Item Type:	Article
Uncontrolled Keywords:	Lippia alba, antimicrobial activity, cytotoxic activity, essential oil
Depositing User:	Symplectic Admin
Date Deposited:	03 Nov 2017 13:28
Last Modified:	13 Feb 2024 15:09
DOI:	10.3390/medicines3030022
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3011422