Fulakeza, Joseph RM, Banda, Rachel L, Lipenga, Trancizeo R, Terlouw, Dianne J, Nkhoma, Standwell C and Hodel, Eva Maria 
ORCID: 0000-0001-5821-1685
(2018)
Comparison of Two Genotyping Methods for Distinguishing Recrudescence from Reinfection in Antimalarial Drug Efficacy/Effectiveness Trials.
, United States.
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Abstract
Genotyping of allelic variants of <i>Plasmodium falciparum</i> merozoite surface proteins 1 and 2 (<i>msp-1</i> and <i>msp-2</i>), and the glutamate-rich protein is the gold standard for distinguishing reinfections from recrudescences in antimalarial drug trials. We compared performance of the recently developed 24-single-nucleotide polymorphism (SNP) Barcoding Assay against <i>msp-1</i> and <i>msp-2</i> genotyping in a cluster-randomized effectiveness trial of artemether-lumefantrine and dihydroartemisinin-piperaquine in Malawi. Rates of recrudescence and reinfection estimated by the two methods did not differ significantly (Fisher's exact test; <i>P</i> = 0.887 and <i>P</i> = 0.768, respectively). There was a strong agreement between the two methods in predicting treatment outcomes and resolving the genetic complexity of malaria infections in this setting. These results support the use of this SNP assay as an alternative method for correcting antimalarial efficacy/effectiveness data.
| Item Type: | Conference or Workshop Item (Unspecified) |
|---|---|
| Uncontrolled Keywords: | Humans, Plasmodium falciparum, Malaria, Falciparum, Recurrence, Artemisinins, Quinolines, Protozoan Proteins, Merozoite Surface Protein 1, Antigens, Protozoan, Drug Combinations, Antimalarials, Treatment Outcome, Cluster Analysis, Gene Expression, Genotype, Polymorphism, Single Nucleotide, Child, Malawi, Female, Male, Merozoites, Genotyping Techniques, Artemether, Lumefantrine Drug Combination |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 15 Nov 2017 15:42 |
| Last Modified: | 19 Jan 2023 06:50 |
| DOI: | 10.4269/ajtmh.18-0002 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3011908 |
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