Extracellular Vesicles of Hypoxic Adipocytes and Obese Subjects Reduce Insulin-stimulated Glucose Uptake



Mleczko, Justyna, Ortega, Francisco, Falcon-Perez, Juan Manuel, Wabitsch, Martin, Fernandez-Real, Jose Manuel and Mora, Silvia
(2018) Extracellular Vesicles of Hypoxic Adipocytes and Obese Subjects Reduce Insulin-stimulated Glucose Uptake. Molecular Nutrition and Food Research, 62 (5). 1700917-.

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Abstract

Scope We investigate the effects of extracellular vesicles (EVs) obtained from in vitro adipocyte cell models and from obese subjects on glucose transport and insulin responsiveness. Methods and results EVs are isolated from the culture supernatant of adipocytes cultured under normoxia, hypoxia (1% oxygen), or exposed to macrophage conditioned media (15% v/v). EVs are isolated from the plasma of lean individuals and subjects with obesity. Cultured adipocytes are incubated with EVs and activation of insulin signalling cascades and insulin‐stimulated glucose transport are measured. EVs released from hypoxic adipocytes impair insulin‐stimulated 2‐deoxyglucose uptake and reduce insulin mediated phosphorylation of AKT. Insulin‐mediated phosphorylation of extracellular regulated kinases (ERK1/2) is not affected. EVs from individuals with obesity decrease insulin stimulated 2‐deoxyglucose uptake in adipocytes (p = 0.0159). Conclusion EVs released by stressed adipocytes impair insulin action in neighboring adipocytes.

Item Type: Article
Uncontrolled Keywords: adipocyte, exosome, glucose transport, insulin
Depositing User: Symplectic Admin
Date Deposited: 22 Feb 2018 10:55
Last Modified: 16 Mar 2024 20:30
DOI: 10.1002/mnfr.201700917
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3014623

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