The effect of surface charge on the thermal stability and ice recrystallization inhibition activity of antifreeze protein III (AFP III)

Deller, RC ORCID: 0000-0002-5812-583X, Carter, BM, Zampetakis, I, Scarpa, F and Perriman, AW (2018) The effect of surface charge on the thermal stability and ice recrystallization inhibition activity of antifreeze protein III (AFP III). BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 495 (1). pp. 1055-1060.

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Abstract

The aim of this study was to examine the effect of chemical cationization on the structure and function of antifreeze protein III (AFP III) over an extreme temperature range (-40°C to +90°C) using far-UV synchrotron radiation circular dichroism (SRCD) and ice recrystallization inhibition (IRI) assays. Chemical cationization was able to produce a modified AFP III with a net cationic charge at physiological pH that had enhanced resistance to denaturation at elevated temperatures, with no immediate negative impact on protein structure at subzero temperatures. Furthermore, cationized AFP III retained an IRI activity similar to that of native AFP III. Consequently, chemical cationization may provide a pathway to the development of more robust antifreeze proteins as supplementary cryoprotectants in the cryopreservation of clinically relevant cells.

Item Type:	Article
Uncontrolled Keywords:	Antifreeze protein, Synchrotron radiation circular dichroism, Ice recrystallization, Thermal stability, Cryopreservation
Depositing User:	Symplectic Admin
Date Deposited:	20 Mar 2018 07:19
Last Modified:	19 Jan 2023 06:38
DOI:	10.1016/j.bbrc.2017.11.073
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3019218