Nanoscale Visualization of Bacterial Microcompartments Using Atomic Force Microscopy

Rodriguez-Ramos, Jorge, Faulkner, Matthew ORCID: 0000-0002-0578-388X and Liu, Lu-Ning ORCID: 0000-0002-8884-4819 (2018) Nanoscale Visualization of Bacterial Microcompartments Using Atomic Force Microscopy. In: NANOSCALE IMAGING: METHODS AND PROTOCOLS. Methods in Molecular Biology, 1814 . Springer New York, pp. 373-383. ISBN 978-1-4939-8590-6

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Abstract

Bacterial microcompartments (BMCs) are polyhedral protein organelles in many prokaryotes, playing significant roles in metabolic enhancement. Due to their self-assembly and modularity nature, BMCs have gained increased interest in recent years, with the intent of constructing new nanobioreactors and scaffolding to promote cellular metabolisms and molecule delivery. In this chapter, we describe the technique of atomic force microscopy (AFM) as a method to study the self-assembly dynamics and physical properties of BMCs. We focus on the sample preparation, the measurement procedure, and the data analysis for high-speed AFM imaging and nanoindentation-based spectroscopy, which were used to determine the assembly dynamics of BMC shell proteins and the nanomechanics of intact BMC structures, respectively. The described methods could be applied to the study of other types of self-assembling biological organelles.

Item Type:	Book Section
Uncontrolled Keywords:	Atomic force microscopy, High-speed AFM, Force spectroscopy, Nanoindentation, Bacterial microcompartment, Carboxysome, Self-assembly, Nanomechanics
Depositing User:	Symplectic Admin
Date Deposited:	04 May 2018 08:36
Last Modified:	15 Mar 2024 05:00
DOI:	10.1007/978-1-4939-8591-3_22
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3020894