Gamma irradiation of electrospun poly(e-caprolactone) fibers affects material properties but not cell response



Bosworth, LA ORCID: 0000-0002-6726-4663, Gibb, A and Downes, S
(2012) Gamma irradiation of electrospun poly(e-caprolactone) fibers affects material properties but not cell response. JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 50 (12). pp. 870-876.

Access the full-text of this item by clicking on the Open Access link.

Abstract

<jats:title>Abstract</jats:title><jats:p>Fabrication of electrospun fibrous scaffolds as future medical devices is being widely researched, with particular emphasis given to their material properties and effect on cell response and differentiation. However, the vast majority of these scaffolds are sterilized via nonmedically approved methods, including submersion in ethanol and exposure to UV light. Although these techniques are adequate for laboratory‐based research, they are not sufficient for human implantation. In this case, regulatory approved, medical grade sterilization is required. In this study, we report the effects of gamma irradiation, a regulatory approved technique, on electrospun poly(ε‐caprolactone) fibers. Fabricated fibers were separately subjected to different dosages of irradiation ranging from 0 to 45 kGy and then assessed for their physicochemical properties. Gamma irradiation affected fiber properties irrespective of dosage. A dose‐dependent decrease in polymer molecular weight was observed and an increase in melting point and crystallinity reported. Similarly, irradiation had a significant effect on mechanical properties with greatest decrease in tensile strength (68%) for fibers exposed to 40 kGy. The method of sterilization had no effect on cell response. Seeded tenocytes attached to all fibers and elongated parallel to the underlying fiber direction. The results demonstrate the importance of incorporating medical grade sterilization procedures early in the research projects time line to assist translation from bench to clinic. © 2012 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2012</jats:p>

Item Type: Article
Uncontrolled Keywords: biomaterials, electrospinning, fiber, gamma irradiation, mechanical properties, molecular weight distribution, molar mass distribution, sterilization
Depositing User: Symplectic Admin
Date Deposited: 13 Aug 2018 06:33
Last Modified: 31 Oct 2023 02:25
DOI: 10.1002/polb.23072
Open Access URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/po...
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3024872