<i>Ximenia americana</i> heteropolysaccharides ameliorate inflammation and visceral hypernociception in murine caerulein-induced acute pancreatitis: Involvement of CB2 receptors

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Sales da Silva-Leite, Kaira Emanuella, Fernandes Bezerra Girao, Deysen Kerlla, Pires, Alana de Freitas, Assreuy, Ana Maria S, Feitosa de Moraes, Pedro Almir, Cunha, Arcelina Pacheco, Pontes Silva Ricardo, Nagila Maria, Criddle, David Neil ORCID: 0000-0003-2952-8450, Loiola Ponte de Souza, Marcellus Henrique, Pereira, Maria Goncalves et al (show 1 more authors) and Gomes Soares, Pedro Marcos (2018) <i>Ximenia americana</i> heteropolysaccharides ameliorate inflammation and visceral hypernociception in murine caerulein-induced acute pancreatitis: Involvement of CB2 receptors. BIOMEDICINE & PHARMACOTHERAPY, 106. pp. 1317-1324.

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Official URL: http://dx.doi.org/10.1016/j.biopha.2018.07.067

Abstract

<h4>Background</h4>This study aimed to investigate and characterize the anti-inflammatory and anti-hypernociceptive effects of the total polysaccharides of X. americana (TPL-Xa) bark in a mouse model of acute pancreatitis-induced by caerulein and the potential involvement of cannabinoid receptors.<h4>Methods</h4>TPL-Xa was characterized by<sup>1</sup>H and <sup>13</sup>C NMR spectroscopy. Animals received TPL-Xa (10 mg/kg, i.v.) 30 min before and after caerulein (50 μg/kg, 10×, i.p.) administration. To evaluate the involvement of cannabinoid receptors, AM281 (3 mg/kg, s.c.) and AM630 (1 mg/kg, s.c.) were administered 30 min before TPL-Xa. Plasma levels of amylase and lipase, pancreatic myeloperoxidase (MPO), histology, visceral hypernociception and motor coordination were evaluated 11 and 24 h after acute pancreatitis (AP) induction.<h4>Results</h4>TPL-Xa, containing a heteropolysaccharide composed of glucose, galactose, arabinose, rhamnose, fucose and galacturonic acid, reduced amylase and lipase levels, MPO activity, acinar cell necrosis, edema and neutrophil infiltration. TPL-Xa increased the threshold of visceral hypernociception, an effect reversed by AM630, an antagonist of cannabinoid receptor type 2 (CB2). In addition, TPL-Xa did not alter the animals' motor coordination.<h4>Conclusions</h4>TPL-Xa contains heteropolysaccharides that inhibit inflammation and hypernociception in the experimental model of caerulein-induced AP, by a mechanism involving type CB2 receptors.

Item Type:	Article
Uncontrolled Keywords:	Pancreas inflammatory nociception, Medicinal plant polysaccharides, Structural characterization, Cannabinoid receptors
Depositing User:	Symplectic Admin
Date Deposited:	18 Oct 2018 07:50
Last Modified:	07 Feb 2024 15:16
DOI:	10.1016/j.biopha.2018.07.067
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3027104