Topological Characterization of Human and Mouse m<SUP>5</SUP>C Epitranscriptome Revealed by Bisulfite Sequencing



Wei, Zhen, Panneerdoss, Subbarayalu, Timilsina, Santosh, Zhu, Jingting, Mohammad, Tabrez A, Lu, Zhi-Liang ORCID: 0000-0002-3442-1415, de Magalhaes, Joao Pedro ORCID: 0000-0002-6363-2465, Chen, Yidong, Rong, Rong, Huang, Yufei
et al (show 2 more authors) (2018) Topological Characterization of Human and Mouse m<SUP>5</SUP>C Epitranscriptome Revealed by Bisulfite Sequencing. INTERNATIONAL JOURNAL OF GENOMICS, 2018. 1351964-.

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Abstract

<h4>Background</h4>Compared with the well-studied 5-methylcytosine (m<sup>5</sup>C) in DNA, the role and topology of epitranscriptome m<sup>5</sup>C remain insufficiently characterized.<h4>Results</h4>Through analyzing transcriptome-wide m<sup>5</sup>C distribution in human and mouse, we show that the m<sup>5</sup>C modification is significantly enriched at 5' untranslated regions (5'UTRs) of mRNA in human and mouse. With a comparative analysis of the mRNA and DNA methylome, we demonstrate that, like DNA methylation, transcriptome m<sup>5</sup>C methylation exhibits a strong clustering effect. Surprisingly, an inverse correlation between mRNA and DNA m<sup>5</sup>C methylation is observed at CpG sites. Further analysis reveals that RNA m<sup>5</sup>C methylation level is positively correlated with both RNA expression and RNA half-life. We also observed that the methylation level of mitochondrial RNAs is significantly higher than RNAs transcribed from the nuclear genome.<h4>Conclusions</h4>This study provides an in-depth topological characterization of transcriptome-wide m<sup>5</sup>C modification by associating RNA m<sup>5</sup>C methylation patterns with transcriptional expression, DNA methylations, RNA stabilities, and mitochondrial genome.

Item Type: Article
Uncontrolled Keywords: Genetics, Human Genome, Generic health relevance
Depositing User: Symplectic Admin
Date Deposited: 26 Nov 2018 10:19
Last Modified: 14 Mar 2024 18:25
DOI: 10.1155/2018/1351964
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3029050