Proteomic differences between native and tissue-engineered tendon and ligament

Kharaz, Yalda A, Tew, Simon R ORCID: 0000-0002-1213-5418, Peffers, Mandy ORCID: 0000-0001-6979-0440, Canty-Laird, Elizabeth G ORCID: 0000-0001-5041-1316 and Comerford, Eithne ORCID: 0000-0002-5244-6042 (2016) Proteomic differences between native and tissue-engineered tendon and ligament. PROTEOMICS, 16 (10). pp. 1547-1556.

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Abstract

Tendons and ligaments (T/Ls) play key roles in the musculoskeletal system, but they are susceptible to traumatic or age-related rupture, leading to severe morbidity as well as increased susceptibility to degenerative joint diseases such as osteoarthritis. Tissue engineering represents an attractive therapeutic approach to treating T/L injury but it is hampered by our poor understanding of the defining characteristics of the two tissues. The present study aimed to determine differences in the proteomic profile between native T/Ls and tissue engineered (TE) T/L constructs. The canine long digital extensor tendon and anterior cruciate ligament were analyzed along with 3D TE fibrin-based constructs created from their cells. Native tendon and ligament differed in their content of key structural proteins, with the ligament being more abundant in fibrocartilaginous proteins. 3D T/L TE constructs contained less extracellular matrix (ECM) proteins and had a greater proportion of cellular-associated proteins than native tissue, corresponding to their low collagen and high DNA content. Constructs were able to recapitulate native T/L tissue characteristics particularly with regard to ECM proteins. However, 3D T/L TE constructs had similar ECM and cellular protein compositions indicating that cell source may not be an important factor for T/L tissue engineering.

Item Type:	Article
Uncontrolled Keywords:	3D tissue engineered ligament construct, 3D tissue engineered tendon constructs, Ligament, Proteomics, Tendon, Technology
Depositing User:	Symplectic Admin
Date Deposited:	18 Jan 2019 14:25
Last Modified:	19 Jan 2023 01:08
DOI:	10.1002/pmic.201500459
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3030733

Available Versions of this Item

• Proteomic differences between native and tissue-engineered tendon and ligament. (deposited 26 Apr 2016 15:49)
  • Proteomic differences between native and tissue-engineered tendon and ligament. (deposited 18 Jan 2019 14:25) [Currently Displayed]