Complete enzyme set for chlorophyll biosynthesis in Escherichia coli

Chen, Guangyu E, Canniffe, Daniel P ORCID: 0000-0002-5022-0437, Barnett, Samuel FH, Hollingshead, Sarah, Brindley, Amanda A, Vasilev, Cvetelin, Bryant, Donald A and Hunter, C Neil (2018) Complete enzyme set for chlorophyll biosynthesis in Escherichia coli. SCIENCE ADVANCES, 4 (1). eaaq1407-.

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Abstract

Chlorophylls are essential cofactors for photosynthesis, which sustains global food chains and oxygen production. Billions of tons of chlorophylls are synthesized annually, yet full understanding of chlorophyll biosynthesis has been hindered by the lack of characterization of the Mg-protoporphyrin IX monomethyl ester oxidative cyclase step, which confers the distinctive green color of these pigments. We demonstrate cyclase activity using heterologously expressed enzyme. Next, we assemble a genetic module that encodes the complete chlorophyll biosynthetic pathway and show that it functions in <i>Escherichia coli</i>. Expression of 12 genes converts endogenous protoporphyrin IX into chlorophyll a, turning <i>E. coli</i> cells green. Our results delineate a minimum set of enzymes required to make chlorophyll and establish a platform for engineering photosynthesis in a heterotrophic model organism.

Item Type:	Article
Uncontrolled Keywords:	Escherichia coli, Protoporphyrins, Recombinant Proteins, Biosynthetic Pathways, Metabolic Engineering
Depositing User:	Symplectic Admin
Date Deposited:	17 Jan 2019 09:24
Last Modified:	19 Jan 2023 01:06
DOI:	10.1126/sciadv.aaq1407
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3031304