Lum, Fok-Moon, Lin, Cui, Susova, Olga Y, Teo, Teck-Hui, Fong, Siew-Wai, Mak, Tze-Minn, Lee, Linda Kay, Chong, Chia-Yin, Lye, David CB, Lin, Raymond TP et al (show 3 more authors)
(2017)
A Sensitive Method for Detecting Zika Virus Antigen in Patients' Whole-Blood Specimens as an Alternative Diagnostic Approach.
JOURNAL OF INFECTIOUS DISEASES, 216 (2).
pp. 182-190.
Abstract
Background Epidemics caused by the reemergence of Zika virus (ZIKV) warrant the need to develop new diagnostic measures to complement currently used detection methods. In this study, we explored the detection of ZIKV antigen in a defined leukocyte subset from patients’ whole-blood specimens. Methods Whole-blood samples were obtained at the acute and early convalescent phases from ZIKV-infected patients during the Singapore outbreak in August–September 2016. Presence of ZIKV antigen was determined by flow cytometry staining for intracellular ZIKV NS3, using a ZIKV-specific polyclonal antibody. The presence of ZIKV antigen was determined in CD45+CD14+ monocytes. Results Data showed that ZIKV NS3 antigen could be detected in CD45+CD14+ monocytes. The levels of detection were further categorized into 3 groups: high (positivity among >40% of monocytes), moderate (positivity among 10%–40%), and low (positivity among <10%). While a majority of patients showed a decrease in the amount of ZIKV antigen detected at later time points, some patients displayed higher levels as the disease progressed. Conclusions Our data highlights an alternative approach in using flow cytometry as a sensitive method for detecting ZIKV antigen in whole blood. Importantly, it further confirms the role of CD14+ monocytes as an important cellular target for ZIKV infection during the viremic phase.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Zika virus, monocytes, detection, diagnosis |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 06 Feb 2019 10:35 |
| Last Modified: | 19 Jan 2023 01:05 |
| DOI: | 10.1093/infdis/jix276 |
| Open Access URL: | https://doi.org/10.1093/infdis/jix276 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3032349 |
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