Mitochondrial respiratory chain deficiency inhibits lysosomal hydrolysis

Fernandez-Mosquera, Lorena, Yambire, King F, Renata, Couto, Pereyra, Leonardo, Pabis, Kamil, Ponsford, Amy H ORCID: 0000-0002-7178-7862, Diogo, Cátia V, Stagi, Massimiliano ORCID: 0000-0002-5827-902X, Milosevic, Ira and Raimundo, Nuno (2019) Mitochondrial respiratory chain deficiency inhibits lysosomal hydrolysis. Autophagy, 15 (9). pp. 1572-1591.

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Official URL: https://www.tandfonline.com/doi/full/10.1080/15548...

Abstract

Mitochondria are key organelles for cellular metabolism, and regulate several processes including cell death and macroautophagy/autophagy. Here, we show that mitochondrial respiratory chain (RC) deficiency deactivates AMP-activated protein kinase (AMPK, a key regulator of energy homeostasis) signaling in tissue and in cultured cells. The deactivation of AMPK in RC-deficiency is due to increased expression of the AMPK-inhibiting protein FLCN (folliculin). AMPK is found to be necessary for basal lysosomal function, and AMPK deactivation in RC-deficiency inhibits lysosomal function by decreasing the activity of the lysosomal Ca2+ channel MCOLN1 (mucolipin 1). MCOLN1 is regulated by phosphoinositide kinase PIKFYVE and its product PtdIns(3,5)P2, which is also decreased in RC-deficiency. Notably, reactivation of AMPK, in a PIKFYVE-dependent manner, or of MCOLN1 in RC-deficient cells, restores lysosomal hydrolytic capacity. Building on these data and the literature, we propose that downregulation of the AMPK-PIKFYVE-PtdIns(3,5)P2-MCOLN1 pathway causes lysosomal Ca2+ accumulation and impaired lysosomal catabolism. Besides unveiling a novel role of AMPK in lysosomal function, this study points to the mechanism that links mitochondrial malfunction to impaired lysosomal catabolism, underscoring the importance of AMPK and the complexity of organelle cross-talk in the regulation of cellular homeostasis.

Item Type:	Article
Uncontrolled Keywords:	AMPK, calcium, lysosomal Ca2+, lysosomes, MCOLN1, mitochondria, mitochondrial respiratory chain deficiency
Depositing User:	Symplectic Admin
Date Deposited:	13 Feb 2019 11:29
Last Modified:	19 Jan 2023 01:04
DOI:	10.1080/15548627.2019.1586256
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3032707