Kurvits, Lille, Reimann, Ene, Kadastik-Eerme, Liis, Truu, Laura, Kingo, Kulli, Erm, Triin, Koks, Sulev 
ORCID: 0000-0001-6087-6643, Taba, Pille and Planken, Anu
(2019)
Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson's Disease Patients.
FRONTIERS IN NEUROSCIENCE, 13 (JAN).
13-.
Abstract
We report the changed levels of serum amyloid alpha, an immunologically active protein, in Parkinson's disease (PD) patients' peripheral tissues. We have previously shown that <i>Saa-1</i> and <i>-2</i> (serum amyloid alpha-1,-2, genes) were among the top downregulated genes in PD patients' skin, using whole-genome RNA sequencing. In the current study, we characterized the gene and protein expression profiles of skin and blood samples from patients with confirmed PD diagnosis and age/sex matched controls. qRT-PCR analysis of PD skin demonstrated downregulation of <i>Saa-1</i> and <i>-2</i> genes in PD patients. However, the lowered amount of protein could not be visualized using immunohistochemistry, due to low quantity of SAA (Serum Amyloid Alpha, protein) in skin. <i>Saa-1</i> and <i>-2</i> expression levels in whole blood were below detection threshold based on RNA sequencing, however significantly lowered protein levels of SAA1/2 in PD patients' serum were shown with ELISA, implying that SAA is secreted into the blood. These results show that SAA is differentially expressed in the peripheral tissues of PD patients.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | neurodegenarative disease, serum amyloid A (SAA), Parkinson's disease (PD), intrinsically disordered protein (IDP), skin biopsy |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 06 Mar 2019 10:42 |
| Last Modified: | 19 Jan 2023 00:57 |
| DOI: | 10.3389/fnins.2019.00013 |
| Open Access URL: | https://www.frontiersin.org/articles/10.3389/fnins... |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3033810 |
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