Najgebauer, Hanna, Jarnuczak, Andrew E, Varro, Andrea and Sanderson, Christopher M ORCID: 0000-0002-7301-3537
(2019)
Integrative Omic Profiling Reveals Unique Hypoxia Induced Signatures in Gastric Cancer Associated Myofibroblasts.
CANCERS, 11 (2).
E263-.
Text
cancers-11-00263-v2.pdf - Published version Download (3MB) |
Abstract
Although hypoxia is known to contribute to several aspects of tumour progression, relatively little is known about the effects of hypoxia on cancer-associated myofibroblasts (CAMs), or the consequences that conditional changes in CAM function may have on tumour development and metastasis. To investigate this issue in the context of gastric cancer, a comparative multiomic analysis was performed on populations of patient-derived myofibroblasts, cultured under normoxic or hypoxic conditions. Data from this study reveal a novel set of CAM-specific hypoxia-induced changes in gene expression and secreted proteins. Significantly, these signatures are not observed in either patient matched adjacent tissue myofibroblasts (ATMs) or non-cancer associated normal tissue myofibroblasts (NTMs). Functional characterisation of different myofibroblast populations shows that hypoxia-induced changes in gene expression not only enhance the ability of CAMs to induce cancer cell migration, but also confer pro-tumorigenic (CAM-like) properties in NTMs. This study provides the first global mechanistic insight into the molecular changes that contribute to hypoxia-induced pro-tumorigenic changes in gastric stromal myofibroblasts.
Item Type: | Article |
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Uncontrolled Keywords: | cancer-associated myofibroblasts, hypoxia, tumour microenvironment, gene expression, secretome, transcriptomics, proteomics, gastric cancer, omics |
Depositing User: | Symplectic Admin |
Date Deposited: | 28 Mar 2019 15:36 |
Last Modified: | 19 Jan 2023 00:55 |
DOI: | 10.3390/cancers11020263 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3035216 |