A multi-column plate adapter provides an economical and versatile high-throughput protein purification system

Dominguez, Matthew J, Lantz, Benjamin J, Rhode, Rebecca J, Sharp, Zoey L, Finney, Krysten C, Martinez, Valeria Jaramillo and Stollar, Elliott J ORCID: 0000-0001-9895-0335 (2018) A multi-column plate adapter provides an economical and versatile high-throughput protein purification system. PROTEIN EXPRESSION AND PURIFICATION, 152. pp. 84-91.

Text E Stollar - A multi-column plate adapter provides an economical and versatile high-throughput protein purification system - AAM.docx - Author Accepted Manuscript Download (1MB)

Abstract

Protein purification is essential in the study of protein structure and function, and the development of novel therapeutics. Many studies require purifying multiple proteins at once, increasing the demand for improved purification methods. We hypothesized that multiple chromatography columns could be interfaced with a multi-well collection plate for rapid and convenient protein purification without the need of expensive instrumentation. As such, we developed a multi-column plate adapter (MCPA), which provides an economical yet versatile and time efficient, high-throughput protein purification system. The MCPA system simultaneously purified milligrams of different proteins under gravity or under vacuum for faster purification. The MCPA handles up to twenty-four 12 mL columns and multiple MCPA's in sequence allow milligram-scale purification of 96 different samples with relative ease. We also used the MCPA system for large scale affinity purification of four proteins, providing sufficient yields and purity for protein crystallization and biophysical characterization. The MCPA system is ideal for optimizing resin type and volume or any other purification parameter by customizing individual columns during the same purification. The high-throughput and versatile nature of this system should prove to be useful in obtaining adequate amounts of protein for subsequent analyses in any laboratory setting.

Item Type:	Article
Uncontrolled Keywords:	Protein purification, High-throughput, SH3 domain
Depositing User:	Symplectic Admin
Date Deposited:	02 Jul 2019 08:13
Last Modified:	19 Jan 2023 00:38
DOI:	10.1016/j.pep.2018.07.009
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3048195