Recognition of xyloglucan by the crystalline cellulose-binding site of a family 3a carbohydrate-binding module



Hernandez-Gomez, Mercedes C, Rydahl, Maja G, Rogowski, Artur, Morland, Carl, Cartmell, Alan ORCID: 0000-0002-5512-249X, Crouch, Lucy, Labourel, Aurore, Fontes, Carlos MGA, Willats, William GT, Gilbert, Harry J
et al (show 1 more authors) (2015) Recognition of xyloglucan by the crystalline cellulose-binding site of a family 3a carbohydrate-binding module. FEBS LETTERS, 589 (18). pp. 2297-2303.

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Abstract

Type A non-catalytic carbohydrate-binding modules (CBMs), exemplified by CtCBM3acipA, are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β-1,4-glucan containing α-1,6-xylose side chains. CtCBM3acipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3acipA. A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM-polysaccharide interactions is discussed.

Item Type: Article
Uncontrolled Keywords: Carbohydrate-binding module, CBM3a, Xyloglucan, Crystalline cellulose, Plant cell wall
Depositing User: Symplectic Admin
Date Deposited: 11 Sep 2019 12:32
Last Modified: 19 Jan 2023 00:27
DOI: 10.1016/j.febslet.2015.07.009
Open Access URL: http://europepmc.org/backend/ptpmcrender.fcgi?acci...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3054146