A centrosome-localized calcium signal is essential for mammalian cell mitosis

Helassa, Nordine ORCID: 0000-0003-3743-1886, Nugues, Charlotte, Rajamanoharan, Dayani, Burgoyne, RD ORCID: 0000-0002-9219-0387 and Haynes, LP ORCID: 0000-0002-1296-0338 (2019) A centrosome-localized calcium signal is essential for mammalian cell mitosis. The FASEB Journal, 33 (12). pp. 14602-14610.

Access the full-text of this item by clicking on the Open Access link.

Text 2019, Helassa et al, FASEB J.pdf - Published version Download (1MB) | Preview

Abstract

Mitosis defects can lead to premature ageing and cancer. Understanding mitosis regulation therefore has important implications for human disease. Early data suggested that calcium (Ca2+) signals could influence mitosis, but these have hitherto not been observed in mammalian cells. Here, we reveal a prolonged yet spatially restricted Ca2+ signal at the centrosomes of actively dividing cells. Local buffering of the centrosomal Ca2+ signals, by flash photolysis of the caged Ca2+ chelator diazo‐2‐acetoxymethyl ester, arrests mitosis. We also provide evidence that this Ca2+ signal emanates from the endoplasmic reticulum. In summary, we characterize a unique centrosomal Ca2+ signal as a functionally essential input into mitosis.—Helassa, N., Nugues, C., Rajamanoharan, D., Burgoyne, R. D., Haynes, L. P. A centrosome‐localized calcium signal is essential for mammalian cell mitosis.

Item Type:	Article
Uncontrolled Keywords:	GCaMP, regulation, cell division
Depositing User:	Symplectic Admin
Date Deposited:	07 Nov 2019 09:38
Last Modified:	30 Jun 2023 14:03
DOI:	10.1096/fj.201901662R
Open Access URL:	https://www.fasebj.org/doi/10.1096/fj.201901662R
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3060749