Liquid chromatography tandem mass spectrometry for plasma metadrenalines



Davies, SL and Davison, AS ORCID: 0000-0001-5501-4475
(2019) Liquid chromatography tandem mass spectrometry for plasma metadrenalines. CLINICA CHIMICA ACTA, 495. pp. 512-521.

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Abstract

Evidence is accumulating which may result in plasma free metadrenalines (PMets) becoming the preferred test for diagnosing phaeochromocytoma and paraganglioma. Moreover, increased availability and benefits over other analytical methods like liquid chromatography with electrochemical detection and immunoassay are causing liquid chromatography tandem mass spectrometry (LC-MS/MS) to become the method of choice for PMet measurement. This review explores the evidence-base supporting this, and summarises published LC-MS/MS analytical methods for PMet analysis. Key aspects of methods (including SPE extraction, HILIC chromatography, MRM MS-detection and standardisation) are discussed. Common causes of analytical interference (e.g. ion suppression/enhancement, ionic cross talk, in source transformation and isobaric interferences) are outlined to illustrate the importance of sample purification and chromatographic resolution. The importance of supine, fasting sampling and Bayesian interpretation against supine, fasting reference intervals are explained, as well as the importance of age-specific reference intervals for normetadrenaline. Confounding factors like diet, drugs, renal function and acute illness are explored, along with potential strategies to address these (e.g. CKD-specific reference intervals).

Item Type: Article
Uncontrolled Keywords: Plasma metadrenalines, Liquid chromatography tandem mass spectrometry, LC-MS/MS
Depositing User: Symplectic Admin
Date Deposited: 29 May 2020 10:55
Last Modified: 19 Jan 2023 00:20
DOI: 10.1016/j.cca.2019.05.024
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3060778