Next-generation sequencing showing potential leachate influence on bacterial communities around a landfill in China

Rajasekar, Adharsh, Sekar, Raju ORCID: 0000-0002-1182-9004, Medina-Roldan, Eduardo, Bridge, Jonathan, Moy, Charles KS and Wilkinson, Stephen (2018) Next-generation sequencing showing potential leachate influence on bacterial communities around a landfill in China. CANADIAN JOURNAL OF MICROBIOLOGY, 64 (8). pp. 537-549.

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Official URL: http://dx.doi.org/10.1139/cjm-2017-0543

Abstract

The impact of contaminated leachate on groundwater from landfills is well known, but the specific effects on bacterial consortia are less well-studied. Bacterial communities in a landfill and an urban site located in Suzhou, China, were studied using Illumina high-throughput sequencing. A total of 153 944 good-quality reads were produced and sequences assigned to 6388 operational taxonomic units. Bacterial consortia consisted of up to 16 phyla, including Proteobacteria (31.9%-94.9% at landfill, 25.1%-43.3% at urban sites), Actinobacteria (0%-28.7% at landfill, 9.9%-34.3% at urban sites), Bacteroidetes (1.4%-25.6% at landfill, 5.6%-7.8% at urban sites), Chloroflexi (0.4%-26.5% at urban sites only), and unclassified bacteria. Pseudomonas was the dominant (67%-93%) genus in landfill leachate. Arsenic concentrations in landfill raw leachate (RL) (1.11 × 10<sup>3</sup> μg/L) and fresh leachate (FL2) (1.78 × 10<sup>3</sup> μg/L) and mercury concentrations in RL (10.9 μg/L) and FL2 (7.37 μg/L) exceeded Chinese State Environmental Protection Administration standards for leachate in landfills. The Shannon diversity index and Chao1 richness estimate showed RL and FL2 lacked richness and diversity when compared with other samples. This is consistent with stresses imposed by elevated arsenic and mercury and has implications for ecological site remediation by bioremediation or natural attenuation.

Item Type:	Article
Uncontrolled Keywords:	landfill, leachate, bacterial diversity, Pseudomonas, arsenic
Depositing User:	Symplectic Admin
Date Deposited:	29 Nov 2019 13:33
Last Modified:	19 Jan 2023 00:18
DOI:	10.1139/cjm-2017-0543
Open Access URL:	http://shura.shu.ac.uk/20871/1/Bridge-Next-Generat...
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3064058