Altered Bioenergetics of Blood Cell Sub-Populations in Acute Pancreatitis Patients.

Morton, Jack C, Armstrong, Jane A, Sud, Ajay, Tepikin, Alexei V, Sutton, Robert and Criddle, David N ORCID: 0000-0003-2952-8450 (2019) Altered Bioenergetics of Blood Cell Sub-Populations in Acute Pancreatitis Patients. Journal of clinical medicine, 8 (12).

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Abstract

Acute pancreatitis (AP) is a debilitating, sometimes fatal disease, marked by local injury and systemic inflammation. Mitochondrial dysfunction is a central feature of pancreatic damage in AP, however, its involvement in circulating blood cell subtypes is unknown. This study compared mitochondrial bioenergetics in circulating leukocytes from AP patients and healthy volunteers: 15 patients with mild to severe AP were compared to 10 healthy controls. Monocytes, lymphocytes and neutrophils were isolated using magnetic activated cell sorting and mitochondrial bioenergetics profiles of the cell populations determined using a Seahorse XF24 flux analyser. Rates of oxygen consumption (OCR) and extracellular acidification (ECAR) under conditions of electron transport chain (ETC) inhibition ("stress" test) informed respiratory and glycolytic parameters, respectively. Phorbol ester stimulation was used to trigger the oxidative burst. Basal OCR in all blood cell subtypes was similar in AP patients and controls. However, maximal respiration and spare respiratory capacity of AP patient lymphocytes were decreased, indicating impairment of functional capacity. A diminished oxidative burst occurred in neutrophils from AP patients, compared to controls, whereas this was enhanced in both monocytes and lymphocytes. The data demonstrate important early alterations of bioenergetics in blood cell sub-populations from AP patients, which imply functional alterations linked to clinical disease progression.

Item Type:	Article
Depositing User:	Symplectic Admin
Date Deposited:	03 Jan 2020 13:28
Last Modified:	19 Jan 2023 00:11
DOI:	10.3390/jcm8122201
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3068989

Available Versions of this Item

• Altered Bioenergetics of Blood Cell Sub-Populations in Acute Pancreatitis Patients. (deposited 03 Jan 2020 13:28) [Currently Displayed]
  • Altered Bioenergetics of Blood Cell Sub-Populations in Acute Pancreatitis Patients. (deposited 13 Jan 2020 11:07)