Scherrer, Simone, Stephan, Roger, Zumthor, Jon Paulin, Kipar, Anja 
ORCID: 0000-0001-7289-3459 and Seehusen, Frauke
(2019)
Morphological and Molecular Characterization of a New <i>Mycobacterium avium</i> Subsp. <i>paratuberculosis</i> S-Type Strain Genotype in Goats.
FRONTIERS IN VETERINARY SCIENCE, 6.
250-.
|
Text
Morphological and Molecular Characterization of a New Mycobacterium avium Subsp. paratuberculosis S-Type Strain Genotype in .pdf - Published version Download (1MB) | Preview |
Abstract
Paratuberculosis is a chronic bacterial disease of global importance mainly in domestic and wild ruminants, caused by <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP). In goats, paratuberculosis is mostly caused by the "C-type" (cattle) and in a few cases by the "S-type" (sheep) strain of MAP. In 2017, a caprine S-type III isolate with a new VNTR profile was identified in a Swiss alpine region. In 2018, new caprine isolates with the same novel VNTR profile originating from a farm of a close by neighboring valley were analyzed. Here we report on this MAP S-type III outbreak in a Swiss dairy goat farm in which we investigated the pathological changes, distribution and genotype of MAP tissue homogenates. Full necropsy and histological examination were undertaken on two female adult goats with a history of weight loss and intermitting diarrhea. Routine and special stains were applied to characterize the morphological changes. DNA was extracted from 33 different tissue samples and tested for MAP by qPCR targeting IS<i>900</i> and F57. Subtyping was performed, using the variable number tandem repeats (VNTR) and mycobacterial interspersed repetitive units (MIRU) approach. The goats showed moderate to marked emaciation and displayed typical clinical features of paratuberculosis. A moderate granulomatous enteritis and regional lymphadenitis with a small to moderate number of acid-fast bacteria within macrophages was detected. MAP detection was mainly restricted to the gastrointestinal tract, mesenteric and hepatic lymph nodes. Subtyping the S-type isolates using a panel of eight established MIRU-VNTR loci identified a new genotype, INMV 218.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Mycobacterium avium subsp. paratuberculosis, goat, VNTR, S-type MAP, genotyping of tissue homogenates |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 04 Feb 2020 11:34 |
| Last Modified: | 09 Oct 2023 18:04 |
| DOI: | 10.3389/fvets.2019.00250 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3073396 |
Dimensions
Dimensions
