Vaas, Markus, Ni, Ruiqing, Rudin, Markus, Kipar, Anja 
ORCID: 0000-0001-7289-3459 and Klohs, Jan
(2017)
Extracerebral Tissue Damage in the Intraluminal Filament Mouse Model of Middle Cerebral Artery Occlusion.
FRONTIERS IN NEUROLOGY, 8 (MAR).
85-.
Abstract
Middle cerebral artery occlusion is the most common model of focal cerebral ischemia in the mouse. In the surgical procedure, the external carotid artery (ECA) is ligated; however, its effect on the tissue supplied by the vessel has not been described so far. C57BL/6 mice underwent 1 h of transient MCAO (tMCAO) or sham surgery. Multi-spectral optoacoustic tomography was employed at 30 min after surgery to assess oxygenation in the temporal muscles. Microstructural changes were assessed with magnetic resonance imaging and histological examination at 24 h and 48 h after surgery. Ligation of the ECA resulted in decreased oxygenation of the left temporal muscle in most sham-operated and tMCAO animals. Susceptible mice of both groups exhibited increased <i>T</i><sub>2</sub> relaxation times in the affected muscle with histological evidence of myofibre degeneration, interstitial edema, and neutrophil influx. Ligatures had induced an extensive neutrophil-dominated inflammatory response. ECA ligation leads to distinct hypoxic degenerative changes in the tissue of the ECA territory and to ligature-induced inflammatory processes. An impact on outcome needs to be considered in this stroke model.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | middle cerebral artery occlusion, mouse, ischemia, external carotid artery, muscle degeneration |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 07 Feb 2020 11:26 |
| Last Modified: | 19 Jan 2023 00:04 |
| DOI: | 10.3389/fneur.2017.00085 |
| Open Access URL: | http://doi.org/10.3389/fneur.2017.00085 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3073953 |
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