Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb-/- Lupus Prone Mice is Revealed by Phosphoproteomic Analysis

Ondee, Thunnicha, Jaroonwitchawan, Thiranut, Pisitkun, Trairak ORCID: 0000-0001-6677-2271, Gillen, Joseph, Nita-Lazar, Aleksandra, Leelahavanichkul, Asada and Somparn, Poorichaya (2019) Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb-/- Lupus Prone Mice is Revealed by Phosphoproteomic Analysis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20 (6). E1354-.

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Official URL: http://dx.doi.org/10.3390/ijms20061354

Abstract

Dysfunction of FcGRIIb, the only inhibitory receptor of the FcGR family, is commonly found in the Asian population and is possibly responsible for the extreme endotoxin exhaustion in lupus. Here, the mechanisms of prominent endotoxin (LPS) tolerance in FcGRIIb-/- mice were explored on bone marrow-derived macrophages using phosphoproteomic analysis. As such, LPS tolerance decreased several phosphoproteins in the FcGRIIb-/- macrophage, including protein kinase C-β type II (PRKCB), which was associated with phagocytosis function. Overexpression of PRKCB attenuated LPS tolerance in RAW264.7 cells, supporting the role of this gene in LPS tolerance. In parallel, LPS tolerance in macrophages and in mice was attenuated by phorbol 12-myristate 13-acetate (PMA) administration. This treatment induced several protein kinase C families, including PRKCB. However, PMA attenuated the severity of mice with cecal ligation and puncture on LPS tolerance preconditioning in FcGRIIb-/- but not in wild-type cells. The significant reduction of PRKCB in the FcGRIIb-/- macrophage over wild-type cell possibly induced the more severe LPS-exhaustion and increased the infection susceptibility in FcGRIIb-/- mice. PMA induced PRKCB, improved LPS-tolerance, and attenuated sepsis severity, predominantly in FcGRIIb-/- mice. PRKCB enhancement might be a promising strategy to improve macrophage functions in lupus patients with LPS-tolerance from chronic infection.

Item Type:	Article
Uncontrolled Keywords:	intestinal candida, dextran sulfate solution induced colitis, dysbiosis, bacteremia, gut leakage
Depositing User:	Symplectic Admin
Date Deposited:	20 Feb 2020 10:38
Last Modified:	10 Oct 2023 13:19
DOI:	10.3390/ijms20061354
Open Access URL:	https://doi.org/10.3390/ijms20061354
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3075873