Surveying bovine digital dermatitis and non-healing bovine foot lesions for the presence of <i>Fusobacterium necrophorum, Porphyromonas endodontalis</i> and <i>Treponema pallidum</i>.

Staton, Gareth James ORCID: 0000-0003-4100-8597, Sullivan, Leigh Emma, Blowey, Roger W, Carter, Stuart D ORCID: 0000-0002-3585-9400 and Evans, Nicholas James ORCID: 0000-0002-2950-1007 (2020) Surveying bovine digital dermatitis and non-healing bovine foot lesions for the presence of <i>Fusobacterium necrophorum, Porphyromonas endodontalis</i> and <i>Treponema pallidum</i>. The Veterinary record, 186 (14). 450-.

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Official URL: http://dx.doi.org/10.1136/vr.105628

Abstract

<h4>Background</h4>Non-healing bovine foot lesions, including non-healing white line disease, non-healing sole ulcer and toe necrosis, are an increasingly important cause of chronic lameness that are poorly responsive to treatment. Recent studies have demonstrated a high-level association between these non-healing lesions and the <i>Treponema</i> phylogroups implicated in bovine digital dermatitis (BDD). However, a polymicrobial aetiology involving other gram-stain-negative anaerobes is suspected.<h4>Methods</h4>A PCR-based bacteriological survey of uncomplicated BDD lesions (n=10) and non-healing bovine foot lesions (n=10) targeting <i>Fusobacterium necrophorum</i>, <i>Porphyromonas endodontalis, Dichelobacter nodosus</i> and <i>Treponema pallidum/T. paraluiscuniculi</i> was performed.<h4>Results</h4><i>P. endodontalis</i> DNA was detected in 80.0% of the non-healing lesion biopsies (p=<0.001) but was entirely absent from uncomplicated BDD lesion biopsies. When compared to the BDD lesions, <i>F. necrophorum</i> was detected at a higher frequency in the non-healing lesions (33.3% vs 70.0%, respectively), whereas <i>D. nodosus</i> was detected at a lower frequency (55.5% vs 20.0%, respectively). Conversely, <i>T. pallidum/T. paraluiscuniculi</i> DNA was not detected in either lesion type.<h4>Conclusion</h4>The data from this pilot study suggest that <i>P. endodontalis</i> and <i>F. necrophorum</i> should be further investigated as potential aetiological agents of non-healing bovine foot lesions. A failure to detect syphilis treponemes in either lesion type is reassuring given the potential public health implications such an infection would present.

Item Type:	Article
Uncontrolled Keywords:	Animals, Cattle, Porphyromonas endodontalis, Fusobacterium necrophorum, Treponema pallidum, Fusobacterium Infections, Treponemal Infections, Syphilis, Cattle Diseases, DNA, Bacterial, Pilot Projects, Polymerase Chain Reaction, Female, Digital Dermatitis, United Kingdom
Depositing User:	Symplectic Admin
Date Deposited:	06 Mar 2020 08:51
Last Modified:	25 Jan 2024 10:15
DOI:	10.1136/vr.105628
Open Access URL:	https://veterinaryrecord.bmj.com/content/early/202...
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3077889