Biosynthesis and Characterization of Copper Nanoparticles Using <i>Shewanella oneidensis</i>: Application for Click Chemistry



Kimber, Richard L, Lewis, Edward A, Parmeggiani, Fabio, Smith, Kurt, Bagshaw, Heath ORCID: 0000-0001-7209-9132, Starborg, Toby, Joshi, Nimisha, Figueroa, Adriana I, van der Laan, Gerrit, Cibin, Giannantonio
et al (show 5 more authors) (2018) Biosynthesis and Characterization of Copper Nanoparticles Using <i>Shewanella oneidensis</i>: Application for Click Chemistry. SMALL, 14 (10). p. 1703145.

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Abstract

Copper nanoparticles (Cu-NPs) have a wide range of applications as heterogeneous catalysts. In this study, a novel green biosynthesis route for producing Cu-NPs using the metal-reducing bacterium, Shewanella oneidensis is demonstrated. Thin section transmission electron microscopy shows that the Cu-NPs are predominantly intracellular and present in a typical size range of 20-40 nm. Serial block-face scanning electron microscopy demonstrates the Cu-NPs are well-dispersed across the 3D structure of the cells. X-ray absorption near-edge spectroscopy and extended X-ray absorption fine-structure spectroscopy analysis show the nanoparticles are Cu(0), however, atomic resolution images and electron energy loss spectroscopy suggest partial oxidation of the surface layer to Cu<sub>2</sub> O upon exposure to air. The catalytic activity of the Cu-NPs is demonstrated in an archetypal "click chemistry" reaction, generating good yields during azide-alkyne cycloadditions, most likely catalyzed by the Cu(I) surface layer of the nanoparticles. Furthermore, cytochrome deletion mutants suggest a novel metal reduction system is involved in enzymatic Cu(II) reduction and Cu-NP synthesis, which is not dependent on the Mtr pathway commonly used to reduce other high oxidation state metals in this bacterium. This work demonstrates a novel, simple, green biosynthesis method for producing efficient copper nanoparticle catalysts.

Item Type: Article
Uncontrolled Keywords: biosynthesis, click chemistry, copper nanoparticles, Shewanella oneidensis, XANES
Depositing User: Symplectic Admin
Date Deposited: 27 Mar 2020 12:23
Last Modified: 02 Feb 2024 00:06
DOI: 10.1002/smll.201703145
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3080758