Dynamin and perforin are associated with neovascularisation in advanced carotid plaques.

Slevin, Mark ORCID: 0000-0003-3767-4861, Ethirajan, Priya, Molki, Mohammed, Gaffney, John, Kumar, Shant, Kumar, Pat and Krupinski, Jerzy (2008) Dynamin and perforin are associated with neovascularisation in advanced carotid plaques. Frontiers in bioscience : a journal and virtual library, 13 (13). pp. 6515-6519.

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Official URL: http://dx.doi.org/10.2741/3171

Abstract

Intimal plaque neovascularization is associated with the development of symptomatic disease and thrombosis, with new 'leaky' fragile microvessels prone to haemorrhage. Perforin or pore forming protein is involved in vascular cell death by forming pores in target cells. Enzymes, in particular, granzyme B are secreted by immune infiltrates present in inflammatory plaque regions and have been shown to induce endothelial cell apoptosis. Similarly, dynamin-2 is a GTPase which mediates oxidised low density lipoprotein-induced apoptosis and is also required for granzyme B-mediated exocytosis and apoptosis. Our pilot studies identified increased expression of these proteins in complicated atherosclerotic plaques. Here we demonstrate by immunohistochemistry that both proteins are over-expressed in angiogenic regions of complicated carotid plaques. Dynamin-2 was extensively localised around microvessels and in immune infiltrating cells whilst perforin was localised in immune infiltrating cells, endothelial cells and smooth muscle cells. Over-expression of these proteins may contribute to plaque destabilisation by increasing cellular apoptosis in vulnerable atherosclerotic plaques.

Item Type:	Article
Uncontrolled Keywords:	Humans, Carotid Artery Diseases, Carotid Stenosis, Thrombosis, Rupture, Spontaneous, Inflammation, Neovascularization, Pathologic, Dynamin II, Endarterectomy, Carotid, Immunohistochemistry, Perforin
Depositing User:	Symplectic Admin
Date Deposited:	01 May 2020 08:50
Last Modified:	18 Mar 2024 00:13
DOI:	10.2741/3171
Open Access URL:	https://www.bioscience.org/2008/v13/af/3171/fullte...
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3085457