Hypoxia induces rapid changes to histone methylation reprogramming chromatin for the cellular response: Supplementary material

Batie, Michael, Frost, Julianty, Frost, Mark, Wilson, James, Schofield, Pieta ORCID: 0000-0002-6398-2537 and Rocha, Sonia ORCID: 0000-0002-2413-4981 (2019) Hypoxia induces rapid changes to histone methylation reprogramming chromatin for the cellular response: Supplementary material. bioRxiv. p. 513069.

Access the full-text of this item by clicking on the Open Access link.

Official URL: http://dx.doi.org/10.1101/513069

Abstract

Molecular dioxygenases include JmjC-containing histone demethylases and PHD enzymes, but only PHDs are considered to be molecular oxygen sensors in cells. Although, it is known that hypoxia can alter chromatin, whether this is a direct effect on histone demethylases or due to hypoxia induced HIF-dependent transcriptional changes is not known. Here, we report that hypoxia induces a rapid and HIF-independent alteration to a variety of histone methylation marks. Genomic locations of H3K4me3 and H3K36me3 following short hypoxia predict the hypoxia gene signature observed several hours later in cells. We show that KDM5A inactivation mimics hypoxic changes to H3K4me3 in its targets and is required for the cellular response to hypoxia. Our results demonstrate a direct link between oxygen sensing and chromatin changes via KDM inhibition.

Item Type:	Article
Additional Information:	copyright: © 2019, Posted by Cold Spring Harbor Laboratory. The copyright holder for this pre-print is the author. All rights reserved. The material may not be redistributed, re-used or adapted without the author’s permission. file: M:\Zotero\storage\E4GFWQ57\Batie et al. - 2019 - Hypoxia induces rapid changes to histone methylati.pdf;M:\Zotero\storage\RHBTRXNW\513069v1.html
Uncontrolled Keywords:	Genetics, Biotechnology, 1 Underpinning research, 1.1 Normal biological development and functioning
Depositing User:	Symplectic Admin
Date Deposited:	11 May 2020 10:30
Last Modified:	15 Mar 2024 14:51
DOI:	10.1101/513069
Open Access URL:	https://www.biorxiv.org/content/10.1101/513069v1.s...
Related URLs:	Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3086505