Histological3Dreconstruction and<i>in vivo</i>lineage tracing of the human endometrium

Tempest, Nicola ORCID: 0000-0002-5209-2985, Jansen, Marnix, Baker, Ann-Marie, Hill, Christopher J ORCID: 0000-0003-3831-4569, Hale, Mike, Magee, Derek, Treanor, Darren, Wright, Nicholas A and Hapangama, Dharani K ORCID: 0000-0003-0270-0150 (2020) Histological3Dreconstruction and<i>in vivo</i>lineage tracing of the human endometrium. JOURNAL OF PATHOLOGY, 251 (4). pp. 440-451.

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Official URL: http://dx.doi.org/10.1002/path.5478

Abstract

Regular menstrual shedding and repair of the endometrial functionalis is unique to humans and higher-order primates. The current consensus postulates endometrial glands to have a single-tubular architecture, where multi-potential stem cells reside in the blind-ending glandular-bases. Utilising fixed samples from patients, we have studied the three-dimensional (3D) micro-architecture of the human endometrium. We demonstrate that some non-branching, single, vertical functionalis glands originate from a complex horizontally interconnecting network of basalis glands. The existence of a multipotent endometrial epithelial stem cell capable of regenerating the entire complement of glandular lineages was demonstrated by in vivo lineage tracing, using naturally occurring somatic mitochondrial DNA mutations as clonal markers. Vertical tracking of mutated clones showed that at least one stem-cell population resides in the basalis glands. These novel findings provide insight into the efficient and scar-less regenerative potential of the human endometrium. © 2020 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.

Item Type:	Article
Uncontrolled Keywords:	3D reconstruction, lineage tracing, endometrium, regeneration, stem cells
Depositing User:	Symplectic Admin
Date Deposited:	06 Jul 2020 09:11
Last Modified:	06 Oct 2023 15:02
DOI:	10.1002/path.5478
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3092699