Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis.

Lo Monaco, Melissa ORCID: 0000-0002-1799-094X, Gervois, Pascal ORCID: 0000-0002-8320-1320, Beaumont, Joel ORCID: 0000-0001-9197-3673, Clegg, Peter ORCID: 0000-0003-0632-0032, Bronckaers, Annelies ORCID: 0000-0001-8969-873X, Vandeweerd, Jean-Michel and Lambrichts, Ivo ORCID: 0000-0001-7520-0021 (2020) Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis. Cells, 9 (4). E980-.

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Official URL: http://dx.doi.org/10.3390/cells9040980

Abstract

Osteoarthritis (OA) is a degenerative and inflammatory joint disorder with cartilage loss. Dental pulp stem cells (DPSCs) can undergo chondrogenic differentiation and secrete growth factors associated with tissue repair and immunomodulation. Leukocyte- and platelet-rich fibrin (L-PRF) emerges in regenerative medicine because of its growth factor content and fibrin matrix. This study evaluates the therapeutic application of DPSCs and L-PRF in OA via immunomodulation and cartilage regeneration. Chondrogenic differentiation of DPSCs, with or without L-PRF exudate (ex) and conditioned medium (CM), and of bone marrow-mesenchymal stem cells was compared. These cells showed differential chondrogenesis. L-PRF was unable to increase cartilage-associated components. Immature murine articular chondrocytes (iMACs) were cultured with L-PRF ex, L-PRF CM, or DPSC CM. L-PRF CM had pro-survival and proliferative effects on unstimulated and cytokine-stimulated iMACs. L-PRF CM stimulated the release of IL-6 and PGE2, and increased MMP-13, TIMP-1 and IL-6 mRNA levels in cytokine-stimulated iMACs. DPSC CM increased the survival and proliferation of unstimulated iMACs. In cytokine-stimulated iMACs, DPSC CM increased TIMP-1 gene expression, whereas it inhibited nitrite release in 3D culture. We showed promising effects of DPSCs in an in vitro OA model, as they undergo chondrogenesis in vitro, stimulate the survival of chondrocytes and have immunomodulatory effects.

Item Type:	Article
Uncontrolled Keywords:	Leukocytes, Extracellular Matrix, Chondrocytes, Stem Cells, Mesenchymal Stem Cells, Dental Pulp, Animals, Mice, Inbred C57BL, Humans, Osteoarthritis, Nitrites, Dinoprostone, Tumor Necrosis Factor-alpha, RNA, Messenger, Interleukin-6, Culture Media, Conditioned, Stem Cell Transplantation, Cell Differentiation, Cell Proliferation, Cell Survival, Chondrogenesis, Phenotype, Adolescent, Female, Male, Interleukin-1beta, Young Adult, Platelet-Rich Fibrin
Depositing User:	Symplectic Admin
Date Deposited:	14 Jul 2020 10:12
Last Modified:	20 Jan 2024 10:00
DOI:	10.3390/cells9040980
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3093969