Salerno, Anna, Brady, Kyla, Rikkers, Margot, Li, Chao, Caamano-Gutierrez, Eva 
ORCID: 0000-0001-7737-5941, Falciani, Francesco ORCID: 0000-0003-1432-2871, Blom, Ashley W, Whitehouse, Michael R and Hollander, Anthony P ORCID: 0000-0003-2897-3747
(2020)
MMP13andTIMP1are functional markers for two different potential modes of action by mesenchymal stem/stromal cells when treating osteoarthritis.
STEM CELLS, 38 (11).
pp. 1438-1453.
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Abstract
Mesenchymal stem cells (MSCs) have been investigated as a potential injectable therapy for the treatment of knee osteoarthritis, with some evidence of success in preliminary human trials. However, optimization and scale‐up of this therapeutic approach depends on the identification of functional markers that are linked to their mechanism of action. One possible mechanism is through their chondrogenic differentiation and direct role in neo‐cartilage synthesis. Alternatively, they could remain undifferentiated and act through the release of trophic factors that stimulate endogenous repair processes within the joint. Here, we show that extensive in vitro aging of bone marrow‐derived human MSCs leads to loss of chondrogenesis but no reduction in trophic repair, thereby separating out the two modes of action. By integrating transcriptomic and proteomic data using Ingenuity Pathway Analysis, we found that reduced chondrogenesis with passage is linked to downregulation of the FOXM1 signaling pathway while maintenance of trophic repair is linked to CXCL12. In an attempt at developing functional markers of MSC potency, we identified loss of mRNA expression for MMP13 as correlating with loss of chondrogenic potential of MSCs and continued secretion of high levels of TIMP1 protein as correlating with the maintenance of trophic repair capacity. Since an allogeneic injectable osteoar therapy would require extensive cell expansion in vitro, we conclude that early passage MMP13+, TIMP1‐secretinghigh MSCs should be used for autologous OA therapies designed to act through engraftment and chondrogenesis, while later passage MMP13−, TIMP1‐secretinghigh MSCs could be exploited for allogeneic OA therapies designed to act through trophic repair.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | chondrogenesis, mesenchymal stem cells, MMP13, TIMP1, trophic repair |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 30 Jul 2020 13:32 |
| Last Modified: | 18 Jan 2023 23:39 |
| DOI: | 10.1002/stem.3255 |
| Open Access URL: | https://doi.org/10.1002/stem.3255 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3095552 |
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