Biomechanical Effects of Two forms of PGF2α on ex-vivo Rabbit Cornea.

Zhu, Rong, Zheng, XiaoBo, Guo, LiLi, Zhao, YiPing, Wang, Yuan, Wu, Jie, Yu, AYong, Wang, JunJie, Bao, FangJun and Elsheikh, Ahmed ORCID: 0000-0001-7456-1749 (2020) Biomechanical Effects of Two forms of PGF2α on ex-vivo Rabbit Cornea. Current eye research, 46 (4). pp. 452-460.

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Official URL: http://dx.doi.org/10.1080/02713683.2020.1805771

Abstract

PURPOSE:To investigate the biomechanical effects of two synthetic prostaglandin F2α analogues (PGF2α), namely Travoprost and Tafluprost, on the ex-vivo rabbit cornea. MATERIALS AND METHODS:96 eyes of 48 Japanese white rabbits were divided into 3 equal groups randomly; the Travoprost treated group (Tra), the Tafluprost treated group (Taf) and the control group (Co). Eyes in Tra and Taf groups were preserved in storage medium for 10 days with 1:10 Travoprost and Tafluprost diluents, respectively; while the Co eyes were preserved in a similar but PGF2α-free medium. 24 corneas of each group were tested under inflation conditions with up to 30 mmHg posterior pressure. The pressure-deformation data obtained experimentally were used in an inverse analysis process to derive the stress-strain behavior of the tissue, using which the tangent modulus, a direct measure of the tissue's material stiffness, was calculated. The remaining 8 specimens of each group were analyzed using electron microscopy for fibril diameter and interfibrillar spacing. RESULTS:Although the central corneal thickness increased significantly in the three groups after storage (p< 0.01), it was similar in all groups both before (p= 0.598) and after storage (p= 0.181). After treatment with Travoprost and Tafluprost, the corneas exhibited lower tangent modulus (by 29.2% and 29.8%, respectively at 6 kPa stress) and larger stromal interfibril spacing (by 21.9% and 23.6%) compared with the control group. There was no significant change in fibril diameter with either Travoprost or Tafluprost treatment (p= 0.769). CONCLUSIONS:The results demonstrated significant reductions in tangent modulus and increases in interfibrillar spacing, which were of similar magnitudes, with the application of two different forms of PGF2α.

Item Type:	Article
Uncontrolled Keywords:	Prostaglandin, cornea, biomechanics, stromal fibril, inverse analysis
Depositing User:	Symplectic Admin
Date Deposited:	20 Aug 2020 08:56
Last Modified:	18 Jan 2023 23:36
DOI:	10.1080/02713683.2020.1805771
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3098042